Growth of Acer truncatum seedlings in response to the seedling substrate with different kinds of biochar
[Objective]In order to study the effects of different kinds and amount of biochar on the growth and nutrition of Acer truncatum seedlings,the better biochar and its application amount are selected to provide a theoretical reference for the scientific application of biochar in A.truncatum seedlings.[Method]The seedlings were used as experimental objects,and pot experiments were carried out.Conventional light substrate without biochar was taken as the control(ck1).3 types of biochar(rubber biochar,rice husk biochar and bamboo biochar)with addition of 30.0,50.0 and 70.0 g·kg-1 were set up.The growth,biomass,photosynthetic index,root morphology and nutrition of the seedlings under different treatments were determined.The principal component analysis and membership function analysis of the seedlings were used to comprehensively evaluate the indicators and explore the best biochar and addition proportion.[Result]The growth,biomass,root morphological index,photosynthetic index and nutrient content of the seedlings were all promoted by adding biochar to the substrate,the effect of rice husk biochar on seedling height,stem diameter,photosynthetic index and root morphology was better than that of bamboo biochar and rubber biochar.50.0 g·kg-1 rice husk biochar addition proportion was the best for root growth of seedlings,compared with ckl,the total root length,total root surface area,total root volume and average root diameter increased by 98.79％,110.22％,175.00％and 37.50％,respectively.70.0 g·kg-1 rice husk biochar addition proportion had significant effects on chlorophyll,net photosynthetic rate,stomatal conductance,and transpiration rate,compared with ckl,which increased by 50.00％,221.76％,200.00％and 146.91％,respectively.The evaluation index of seedlings with 50.0 g·kg-1 rice husk biochar was the highest(0.665),which was the best amount of biochar.[Conclusion]The addition of biochar to the the substrate could promote the absorption and utilization of nutrients in A.truncatum seedlings.It is suggested that 50.0 g·kg-1 rice husk biochar should be preferentially selected when adding biochar to A.truncatum seedling substrate.[Ch,3 fig.4 tab.31 ref.]
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