Effects of abiotic stress treatments on the transcriptional activity of transposable element-derived TUCP in Phyllostachys edulis
[Objective]Transposable elements(TE),an essential component of eukaryotic genomes are prone to activation under stress when they account for over 63％of the Phyllostachys edulis genome.This study,with an analysis of the expression patterns of transcripts of uncertain coding potential(TUCP)from transposable elements under abiotic stress,is aimed to provide insights into the molecular mechanisms of TEs in stress resistance in Ph.edulis.[Method]First,bioinformatics techniques were employed to investigate the transcriptional characteristics and patterns of TE-TUCPs,and neighboring genes in Ph.edulis under 4 stress treatments:low temperature,high temperature,high salinity,and UV irradiation.Then the reliability of the differentially expressed TE-TUCPs,data derived from the transcriptome was validated using RT-qPCR.[Result]A total of 57 627 TE-TUCPs were identified from the transcripts of Ph.edulis under 4 stress treatments.These TE-TUCPs exhibited specific expression patterns in response to different abiotic stresses.High temperature,high salinity,and UV irradiation promoted differential expression of genes neighboring TE-TUCPs with transcriptional activity,whereas low temperature suppressed such differential expression.[Conclusion]TE-TUCPs were primarily derived from the Ty1/Copia and Ty3/Gypsy superfamilies.The expression potential of genes and that of nearby TE-TUCPs were mutually inhibitory and the transcription of TE-TUCPs was regulated by abiotic stress to modulate the expression of neighboring genes in response to stress.[Ch,7 fig.2 tab.44 ref.]
Phyllostachys edulistransposable elements(TE)transcripts of uncertain coding potential(TUCP)abiotic stresses
万方数据
维普
