Extraction of essential oil from the whole plant,leaves and stem of Sedum clavatum was implemented by microwave assisted hydro-distillation,and analysis were made on their component by gas chromatography-mass spectrometry.The optimal technology for extraction was experimented with single factor and orthogonal design.The result demonstrated that the extraction rate of essential oil from stem was about 4 times higher than that from leaf,and there were 2 same substances in essential oil from stem and the whole plant.The experiment showed the optimal technology for extraction of essential oil from different part of S.clavatum was as follows:solid-liquid ratio of 1:8,soaking time of 60 min,microwave power of 400 W,and extraction time of 3 h,the highest extraction rate reached 0.96%.