Tissue cultured Anoectochilus roxburghii seedlings were transplanted in nursery of Wenzhou,and under Phyllostachy edulis and under mixed forest of Ph.edulis and Cunninghamia lanceolata in Pingyan,Zhejiang province.In November of the current and the 2nd and 3rd year,determinations were implemented on content of polysaccharide and flavonoids,fresh and dry biomass of seedlings.The results showed that the contents of soluble polysaccharide and flavonoids in understory cultivated A.roxburghii were higher than that in the nursery within the first year,and the contents showed a decreasing trend with the increase of years.Fresh weight increased in the nursery was higher than that understory.Dry weight of understorey cultivated seedlings was higher than that in the nursery.