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不同山乌桕株系叶片药用品质和产量特征分析

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本研究统计了 11个育种株系山乌桕Sapium discolor品种在夏季和秋季的株高、产量等 5个表型特征,测定其不同叶龄、不同采收期叶片的总黄酮含量和抗氧化活性,并对总黄酮含量、抗氧化活性等主要指标进行相关性分析,利用层次分析法筛选综合性状最优的品种.结果表明:(1)'庆乌 2号'在两个采收节点较其他品系均表现出更高的产量等表型性状;(2)8月采收的山乌桕叶片的总黄酮含量和抗氧化活性显著高于 11月采收叶片,同期采收的山乌桕老叶较新叶具有更高的总黄酮含量和更好的抗氧化活性;'庆乌 2号'叶片的总黄酮含量显著高于其他样品,而'龙乌 3号'和'庆乌 4号'的抗氧化活性显著高于其他样品;(3)相关性分析和基于层次分析法的综合评分显示,总黄酮含量与产量和抗氧化活性均具显著呈正相关(P<0.05);层次分析法显示'庆乌 2号'综合评分最高.以上结果表明,8月份采收的山乌桕叶片具有更高的产量和更优的药用品质,山乌桕老叶的药用品质优于新叶;山乌桕叶片总黄酮含量与产量和抗氧化活性呈极显著正相关(P<0.01);'庆乌 2号'具有最好的综合性状,适用于后续新品种选育.
Analysis of the Medicinal Quality and Yield Characteristics of Leaves from Different Populations of Sapium discolor
Objective:To establish a method for determining the total flavonoid content and antioxidant activity of Sapium discolor,correlation analy-sis was conducted based on yield,and the analytic hierarchy process(AHP)was employed to evaluate the different varieties of Sapium discolor.Meth-ods:The total flavonoid content in Sapium discolor cultivars during summer and autumn was analyzed,alongside six phenotypic characteristics such as plant height and yield.The ABTS and DPPH methods were used to determine the antioxidant activity of different batches of Sapium discolor extracts.The varieties with the best comprehensive traits were selected using the analytic hierarchy process.Results:(1)'Qingwu 2'exhibited higher yield and better phenotypic traits compared to other varieties at two harvesting periods.(2)From the perspective of total flavonoid content and antioxidant activi-ty,Sapium discolor leaves harvested in August had higher total flavonoid content and better antioxidant activity.The older leaves contained higher to-tal flavonoid content and showed better antioxidant activity compared to the new leaves.'Qingwu 2'had higher total flavonol content than other vari-eties,while'Longwu 3'and'Qingwu 4'showed higher antioxidant activity.(3)Correlation analysis and AHP-based scores revealed that total flavonol content was positively correlated with yield and antioxidant activity.The AHP results indicated that'Qingwu 2'had the highest comprehensive score.Conclusion:The leaves harvested in summer have higher yields and better medicinal quality,with older leaves showing superior medicinal properties compared to new leaves.There is a significant positive correlation between total flavonoid content,yield,and antioxidant activity.'Qingwu 2'possess-es the best comprehensive traits and is suitable for the breeding of new varieties.

Sapium discolorTotal flavonoidsAntioxidant activityAnalytic Hierarchy Process

蔡龙、梁雅倩、陈杏林、练发良、刘小燕、洪震、孙健、陈世通、刘跃钧

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浙江省中药研究所有限公司,浙江 杭州 310023

云和县石塘镇林业工作站,浙江 云和 323604

龙泉市林业科学研究所,浙江 龙泉 323700

华东药用植物园科研管理中心,浙江 丽水 323000

青田县林业局,浙江 青田 323900

丽水市林业技术推广总站,浙江 丽水 323000

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山乌桕 总黄酮 抗氧化活性 层次分析法

2024

浙江林业科技
浙江省林业科学研究院 浙江省林学会 浙江省林业科技情报中心

浙江林业科技

影响因子:0.483
ISSN:1001-3776
年,卷(期):2024.44(5)