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二年残孔菌胞外多糖组分分析及其在卷烟中的应用

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为开发新型天然菌物香料,利用Sepharose CL-6B凝胶柱对二年残孔菌胞外多糖(EPS)进行分离纯化,并利用红外光谱(IR)、气质联用仪(GC/MS)对EPS的特征性结构和单糖组分进行分析。同时,将EPS进行烟草薄片加香实验,采用同时蒸馏萃取和GC/MS分析了试验组与空白组卷烟中香味物质的变化。结果表明,二年残孔菌EPS为单组分多糖,主要单糖组成为葡萄糖51.45%,甘露糖30.30%,半乳糖15.51%。 GC/MS分析结果显示,将EPS用于制烟后,烟叶中酮类、醛类和呋喃类的含量增加,酚类、氮杂环类的含量显著下降,这有利于减轻烟气刺激性、改善和修饰卷烟香气,进而提高烟气整体品质。
Component analysis of extracellular polysaccharide by Abortiporus biennis and its application in cigarette aroma
In order to develop new natural fungal perfume , the fermented extracellular polysaccharides (EPS) from Abortiporus biennis were purified by Sepharose CL-6B.The structure and monosaccharide composition of EPS were investigated by infrared spectroscopy (IR) and GC-MS analysis.After addition of EPS into tobacco conduction , the changes of flavoring substances in cigarettes were compared with control by simultaneous distillation and GC /MS.It was shown that EPS was monocomponent with the monosaccharide composition of 51.45% glucose, 30.30%mannose and 15.51%galactose.With application of EPS in tobacco production , the contents of ketones, aldehydes and furans increased in the cigarettes , while the contents of phenols and heterocyclic compounds decreased significantly.The treatments with EPS could reduce stimulation of smoking , and improve the aroma of cigarette to ameliorate the quality of smoking.

Abortiporus biennisfermentationextracellular polysaccharidesaroma components

曹健、张惠杰、许春平

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河南工业大学 生物工程学院,河南 郑州450001

中原工学院,河南 郑州 450007

郑州轻工业学院,河南 郑州 450000

二年残孔菌 发酵 胞外多糖 香气成分

国家人社部“留学人员科技项目择优支持计划”

豫留学函[2010]16号

2014

浙江农业学报
浙江省农业科学院 浙江省农学会

浙江农业学报

CSTPCDCSCD北大核心
影响因子:0.765
ISSN:1004-1524
年,卷(期):2014.(2)
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