大巴山粉葛组织培养技术
Tissues culture of Pueraria lobata (wild) Ohwi Dabashan
华晓琴 1刘高亮 2张庆辉 2张鲁 1章婷 1钟宇1
作者信息
- 1. 四川农业大学 林学院，四川 成都 611130
- 2. 四川省三台县林业局，四川 三台 621100
- 折叠
摘要
为了实现大巴山粉葛Pueraria lobata （ wild） Ohwi Dabashan种苗的规模化快速繁殖，以大巴山粉葛茎段为外植体，研究了灭菌时间、MS培养基大量元素、植物生长调节剂对大巴山粉葛组织培养过程中消毒、初代培养、增殖培养及生根培养的影响。结果表明，（1）先用75％乙醇消毒20 s，再用0.1％氯化汞消毒10 min，成活率达67.56％。（2）初代培养基为1／2 MS ＋0.5 mg· L－16-BA ＋0.3 mg· L－1 IBA，腋芽萌发率可达92.2％；（3）增殖培养基为1／2 MS＋1.0 mg· L－16-BA＋0.05 mg· L－1 IBA，增殖系数约5.0；（4）生根培养基为1／2 MS＋0.01 mg· L－1 NAA＋0.1 mg· L－1 IBA，生根率达96.03％。
Abstract
In order to realize the rapid propagation of Pueraria lobata(wild) Ohwi Dabashan, the effects of steriliza-tion time, macroelement in MS medium and plant growth regulators on disinfection , initial culture, multiplication culture and rooting culture of Pueraria lobata( wild) Ohwi Dabashan were studied by tissues culture , using its stem with axillary bud as explants .The results showed that:(1) The explants were sterilized in 75%alcohol and 0.1%HgCl2 for 20 s and 10 min, the survival rate reached to 67.56%.(2) The medium for the initial culture was 1/2 MS+0.5 mg· L-1 6-BA+0.3 mg· L-1 IBA, the percentage of axillary bud was up to 92.2%.(3) The optimum multipli-cation medium was 1/2 MS+1.0 mg· L-1 6-BA+0.05 mg· L-1 IBA, multiplication coefficient reached to 5.0.(4) The rooting medium was 1/2 MS+0.01 mg· L-1 NAA+0.1 mg· L-1 IBA, with rooting rate 96.03%.
关键词
大巴山粉葛/组织培养/腋芽萌发/植物生长调节剂Key words
Pueraria lobata( wild) Ohwi Dabashan/tissues culture/axillary bud sprouting/plant growth regulator引用本文复制引用
基金项目
四川省育种攻关项目(2011NA0098-10)
出版年
2016
国家科技期刊平台
NSTL
维普
万方数据