Interaction analysis between rice ubiquitin ligase D3 and the disease resistance associated protein VOZ2
The tiller dwarf gene dwarf-3(D3)is an important node gene in strigolactone signal transduction in rice.It has been reported that the MAX2 gene in Arabidopsis thaliana is homologous to D3,and MAX2 is involved in the defense response of A.thaliana.In this study,the yeast two-hybrid library was screened with D3 protein as bait,and it was found that there was a potential interaction between rice disease resistance-related protein vascular plant one-zinc finger protein 2(VOZ2)and D3.The interaction between D3 and VOZ2 was confirmed by yeast two-hybrid validation.Real-time quantitative PCR experiments showed that the expression of VOZ2 gene was significantly in-duced at the transcriptional level after inoculation with Xanthomonas oryzae pv.oryzae.Subcellular co-localization experiment using rice protoplasts showed that D3 and VOZ2 were co-localized in the nucleus.Bimolecular fluores-cence complementation experiments showed that D3 and VOZ2 produced strong fluorescence in the nucleus and cyto-plasm of tobacco mesophyll cells,further confirming the interaction between D3 and VOZ2.The study result laid a foundation for further exploring of the function and molecular mechanism of D3 and VOZ2 in rice disease resistance and defense response.
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