Histological study on the effect of high temperature stress on liver tissue in Larimichthys poly-actis
In order to explore the effect of high temperature stress on the liver tissue structure and apoptosis of Larim-ichthys polyactis,the rearing temperature was raised from 15℃ to 31℃ by altering the water temperature in the tank at a constant rate of 2℃·h-1,and maintained to 96 h,recorded as the test group.At the same time,a control group rearing under the temperature of 15℃ was set.At the 0,6,12,24,48,72,96 h after the rearing tempera-ture of the test group reached 31℃ ,the liver tissue of the test and control group were collected to investigate the his-tological changes and apoptosis of the liver tissues through the methods of tissue sections and terminal-deoxynucleoti-dyl transferase mediated dUTP nick end labeling(TUNEL),respectively.The results showed that the liver morphol-ogy of microstructure and ultrastructure were similar to those of the control group after 0 h treatment at 31℃,and there was no significant change.However,with the extension of high temperature treatment time,liver tissue damage gradually worsened.After 96 h of treatment,the liver showed serious damage,including edema,disappearance or e-ven dissolution of nuclei,local necrosis of liver tissue,and destruction of the original structure of liver cells.In addi-tion,with the continuation of high temperature stress,the phenomenon of liver cell apoptosis gradually aggravated.The results showed that high temperature stress caused a certain degree of damage to the liver tissue of L.polyactis and induced a large number of liver cell apoptosis.The results of the present study clarified the effect of high temper-ature stress on the liver tissue of L.polyactis,which is of great significance for the rearing management of L.polyac-tis to avoid the occurrence of liver disease.
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