Natural products screening with resistance to honeybee viruses
In order to identify natural products with anti-honeybee virus effects,9 natural products(thymol,artemis-inin,catalpol,piperine,theobromine,amygdalin,caffeic acid,hesperidin and ρ-coumaric acid)were tested in lab conditions.RT-PCR was used to measure the inhibitory effect of the 9 natural products on honeybee viruses after 7 days.Additionally,the effects of the natural products on the expression of honeybee immunity-related genes were also determined.The results of the exogenous inoculation of Israeli acute paralysis virus(IAPV)virus suggest that feed-ing honeybees with 10 μmol·L-1 thymol for 7 days can significantly reduce the viral load of IAPV and Sacbrood virus(SBV).Without external vaccination of the virus,after feeding with10 μmol·L-1 thymol for7 days,the viral load of IAPV and SBV carried by honeybees was significantly reduced compared with the control group,and the transcrip-tion level of vitellogenin gene was significantly increased.After screening,it was found that honeybees fed with 10 μmol·L-1 thymol can achieve good antiviral effects after 7 days,and thymol can become a potential component in the development of anti-honeybee virus drugs.
万方数据
维普
