Evaluation on nutritional value of Flammulina filiformis cultivation substrate with different cultivation time
In order to evaluate the effect of different cultivation time on the nutritional value of Flammulina filiformis cultivation substrate,the samples of the cultivation substrate of F.filiformis were collected on the 0th,9th,14th,18th,22nd,29th,36th,and 51st days of cultivation.The contents of nutritional ingredients and amino acid(AA)composition of these samples were determined.Besides,the nylon bag technique was adopted to determine the dry matter degradability after in vivo digestion in the rumen for 0,2,4,8,12,24,48,72 h,respectively,as well as the degradation rate of nutritional ingredients after in vivo digestion for 24,48 h,respecitvely.It was shown that,compared with the substrate before cultivation,the contents of crude protein,essential amino acids and total amino acids in the substrate cultivated for 29 d were significantly(P<0.05)increased to 8.96%,0.47%,1.01%,re-spectively,yet the aicd detergent lignin content was significantly decreased to the lowest level(7.72%)under the experiement conditions.For the substrate cultivated for 51 d,the hemicellulose content was the highest(31.37%),and the dry matter degradability was relatively high.In general,F.filiformis cultivation substrate could be used as the feed resource for ruminants,and the optimized cultivation time was 29 d.