Development of SSR molecular markers and application of fingerprint database based on whole genome of Stropharia rugosoannulata
Based on experimental validation and bioinformatics methods,SSR analysis result were exploited from the genomic of Stropharia rugosoannulata and 13 strains.In the meantime,100 SSR were selected for primer design and experimental verification.In order to provide a theoretical basis for the germplasm resources identification,variety selection and molecular marker-assisted breeding of S.rugosoannulata.The results showed that a total of 2 124 se-quences of 6 SSR types were retrieved.The main type of trinucleotide(P3)was 704,accounting for 33.15%of the total SSR number.Pentanucleotide and hexanucleotide repeat types were more,the total number accounted for 14.59%of the total SSR number.16 pairs of primers were obtained by verification test,three primers with high pair property,repeatability and specificity were obtained from the validation experiment,which were DQGSSR020,DQGSSR031 and DQGSSR077.At the same time,the DNA fingerprint coding of 16 pairs of primers was constructed and the 32-digit molecular fingerprint database codes of 13 S.rugosoannulata strains were obtained.
Stropharia rugosoannulatagenomeSSR molecular markers development
万方数据
维普
