Identification and expression analysis of sucrose transporter SUT family in watermelon in fruit development and stress responses
Sucrose transporter(SUTs)gene,as the main active transport vector of sucrose,plays a key role in the transport of allotment assimilates from source tissue to sink tissue.Although the characteristics and biological func-tions of SUTs have been well studied in higher plants,the SUT gene family has not been identified in watermelon.In this study,four ClSUT genes were identified in watermelon genome,and their gene and protein structure,conserved motifs,subcellular localization,chromosome distribution,evolutionary relationships and promoter cis-elements were comprehensively analyzed.Gene structure analysis showed that ClSUT3 and ClSUT4 grouped into subgroup Ⅰ and each only contained one intron.All ClSUT proteins were predicted to be localized in the plasma membrane.All ClSUT proteins contain a conserved MFS domain corresponding to five motifs.Phylogenetic analysis revealed that SUT proteins in Arabidopsis,rice and watermelon were divided into 5 subgroups.ClSUTs in watermelon have more closely relationship with SUT proteins in Arabidopsis and were clustered in subgroupⅠ,ⅡandⅤ.In addition,We also identified several functional cis-elements related to hormone and stress response in the watermelon ClSUT gene promoter.And transcriptome data indicated that watermelon ClSUT genes were variously expressed in response to os-motic and salt stress,which may be involved in watermelon responses to these stresses.
万方数据
维普
