Genetic diversity analysis and fingerprint construction of Dading bitter gourd germplasm re-sources
To achieve rapid and accurate identification of the germplasm resources of the Dading bitter gourd,26 Da-ding bitter gourd germplasm resources including the main cultivars were selected as materials,cluster analysis and fingerprint construction were carried out by screening the high polymorphism simple sequence repeat(SSR)core markers.The results showed that among the 6 SSR motif unit types in the bitter gourd genome,the polymorphism of dinucleotide SSR markers was the best,which was suitable for further screening of SSR core markers.At the genetic similarity coefficient of 0.80,40 SSR markers clustered the 26 Dading bitter gourd germplasm resources into 3 main groups.The first group contained 18 germplasm resources,and the fruit shape was mainly short conical.The second group contained 5 germplasm resources,and the fruit shape was mainly long conical.The third group contained 3 germplasm resources,consisting of materials with fruits similar to those of Dading bitter gourds but containing non-Dading bitter gourd germplasm backgrounds.The 4 SSR core markers,MC05_69594,MC04_50530,MC06_87314 and MC10_146038,were used to effectively identify 26 Dading bitter gourd germplasm resources,and a unique QR code fingerprint was established for each Dading bitter gourd germplasm,among which No.1 and No.3 were suspec-ted to be similar materials.Taken together,the fingerprint of Dading bitter gourd could provide a scientific basis for the germplasm identification and breeding utilization for Dading bitter gourd.
万方数据
维普
