感赤星病与健康烟叶在腈菌唑应用后的叶际微生态

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中文摘要：为了解腈菌唑在防控赤星病时,烟叶叶际微生物菌群结构与代谢功能的差异,采用Illumina高通量测序技术分析了感病与健康烟叶真菌和细菌的菌群结构与多样性特征,采用Biolog-ECO技术分析其代谢功能.结果表明,药前感病烟叶与健康烟叶叶际真菌有链格孢属、谷菌根菌属、Boeremia、枝孢霉属等,其中链格孢属为优势菌属;叶际细菌有科萨克氏菌属、泛菌属、甲基杆菌属等,其中科萨克氏菌属为优势菌属;感病烟叶链格孢属和科萨克氏菌属相对丰度均高于健康烟叶.每667 m2 采用15%腈菌唑微乳剂60 mL处理5、10、15 d时,感病烟叶叶际真菌链格孢属和枝孢霉属相对丰度均降低,附球菌属和亚隔孢壳属相对丰度均先降低后增加;健康烟叶叶际真菌链格孢属、Symmetrospora、亚隔孢壳属的相对丰度均下降.感病烟叶叶际细菌科萨克氏菌属相对丰度在施药5d时降低,10 d和15d时增加,假单胞菌属与之相反;健康烟叶叶际细菌泛菌属、假单胞菌属相对丰度在施药5d和10d时降低,15 d时增加.感病烟叶叶际微生物代谢活性弱于健康烟叶,叶际微生物对除α-丁酮酸、L-苏氨酸和4-羟基苯甲酸外的碳源代谢均较强.腈菌唑应用后,感病与健康烟叶叶际微生物的代谢活性均下降,其代谢活性均随施药时间的延长而逐渐恢复.研究结果揭示了腈菌唑施用不同时期感病与健康烟叶组织叶际微生态变化差异,为腈菌唑防控烟草赤星病的科学应用提供了参考依据.

外文标题：Phyllosphere microecology of brown spot and healthy tobacco leaves after application of my-clobutanil

外文摘要：To understand the differences in the structure and metabolic functions of the phyllosphere microorganisms of tobacco leaves in the control of brown spot by myclobutanil,Illumina high-throughput sequencing and Biolog-ECO technology was conducted to investigate the structure and diversity of phyllospheric microorganisms community and their metabolic function.The results showed that the phyllosphere fungi of the diseased and healthy tobacco leaves in-cluded Alternaria,Archaeorhizomyces,Boeremia,Cladosporium,etc.,of which Alternaria was the dominant genus;the bacteria were Kosakonia,Pantoea and Methylobacterium.Among them,Kosakonia is the dominant genus,and the relative abundance of the Alternaria and Kosakonia was higher in diseased tobacco leaves than in healthy tobacco leaves.The relative abundance of Alternaria and Cladosporium decreased,while the relative abundance of Epicoccum and Didymella decreased and then increased when treated with 60 mL 15%myclobutanil microemulsion per 667 m2 for 5,10 and 15 d;the relative abundance of phyllosphere fungi in healthy tobacco leaves decreased in the genera Alternaria,Symmetrospora,and Didymella.The relative abundance of phyllosphere Kosakonia in diseased tobacco leaves decreased at 5 d of application and increased at 10 and 15 d,while the opposite was true for Pseudomonas.The relative abundance of Pantoea and Pseudomonas decreased at 5 and 10 d and increased at 15 d.The phyllo-spheric microorganism metabolic activity of brown spot-infected leaves was lower than that of healthy tobacco leaves.The metabolism of carbon sources other than α-butyric acid,L-threonine and 4-hydroxybenzoic acid by phyllosphere microorganisms was strong.The metabolic activity of phyllosphere microorganisms in diseased and healthy tobacco leaves were reduced after myclobutanil treatment,and the metabolic activity of phyllospheric microorganisms gradual-ly recovered with the increasing of application time.The results of the study revealed the differences in phyllosphere microecological changes between diseased and healthy tobacco leaves during different periods of myclobutanil applica-tion,providing a reference for the scientific application of myclobutanil in the control of tobacco brown spot.

外文关键词：

tobacco brown spotmyclobutanilphyllosphere microecologyAlternaria sp.metabolic function

作者：

张艺、王丰、蔡刘体、汪汉成、熊晶、陈兴江

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作者单位：

贵州大学农学院,贵州贵阳 550025

贵州省烟草科学研究院,贵州贵阳 550081

贵州省烟草公司毕节市公司,贵州毕节 551700

关键词：

烟草赤星病腈菌唑叶际微生态链格孢属代谢功能

基金：

贵州省"百层次"创新型人才国家自然科学基金国家自然科学基金贵州省科技基金项目中国烟草总公司科技项目中国烟草总公司科技项目

项目编号：

黔科合平台人才-GCC[2022]028-13196055032160522黔科合基础-ZK[2021]重点036110202001035LS-04110202101048LS-08

出版年：

2024

DOI：

10.3969/j.issn.1004-1524.20230242

浙江农业学报

浙江省农业科学院浙江省农学会

浙江农业学报

CSTPCD北大核心

影响因子：0.765

ISSN：1004-1524

年,卷(期)：2024.36(1)

参考文献量6