To understand the differences in the structure and metabolic functions of the phyllosphere microorganisms of tobacco leaves in the control of brown spot by myclobutanil,Illumina high-throughput sequencing and Biolog-ECO technology was conducted to investigate the structure and diversity of phyllospheric microorganisms community and their metabolic function.The results showed that the phyllosphere fungi of the diseased and healthy tobacco leaves in-cluded Alternaria,Archaeorhizomyces,Boeremia,Cladosporium,etc.,of which Alternaria was the dominant genus;the bacteria were Kosakonia,Pantoea and Methylobacterium.Among them,Kosakonia is the dominant genus,and the relative abundance of the Alternaria and Kosakonia was higher in diseased tobacco leaves than in healthy tobacco leaves.The relative abundance of Alternaria and Cladosporium decreased,while the relative abundance of Epicoccum and Didymella decreased and then increased when treated with 60 mL 15%myclobutanil microemulsion per 667 m2 for 5,10 and 15 d;the relative abundance of phyllosphere fungi in healthy tobacco leaves decreased in the genera Alternaria,Symmetrospora,and Didymella.The relative abundance of phyllosphere Kosakonia in diseased tobacco leaves decreased at 5 d of application and increased at 10 and 15 d,while the opposite was true for Pseudomonas.The relative abundance of Pantoea and Pseudomonas decreased at 5 and 10 d and increased at 15 d.The phyllo-spheric microorganism metabolic activity of brown spot-infected leaves was lower than that of healthy tobacco leaves.The metabolism of carbon sources other than α-butyric acid,L-threonine and 4-hydroxybenzoic acid by phyllosphere microorganisms was strong.The metabolic activity of phyllosphere microorganisms in diseased and healthy tobacco leaves were reduced after myclobutanil treatment,and the metabolic activity of phyllospheric microorganisms gradual-ly recovered with the increasing of application time.The results of the study revealed the differences in phyllosphere microecological changes between diseased and healthy tobacco leaves during different periods of myclobutanil applica-tion,providing a reference for the scientific application of myclobutanil in the control of tobacco brown spot.
关键词
烟草赤星病/腈菌唑/叶际微生态/链格孢属/代谢功能
Key words
tobacco brown spot/myclobutanil/phyllosphere microecology/Alternaria sp./metabolic function
维普
万方数据