Cloning of paxillin gene from Macrobrachium nipponense and effect of cadmium stress on its expression
Cadmium(Cd),with strong accumulation and toxicity,is one of the non-essential heavy metal elements,which could cause great harm to aquatic organisms.To better understand the molecular toxicity of cadmium to Macro-brachium nipponense,and provide biomarkers for ecological risk warning and assessment of cadmium pollution in wa-ter environment.In this paper,paxillin gene(MnPXN)of M.nipponense was cloned,and the effect of cadmium stress on its expression was examined.The total length of MnPXN gene is 3 655 bp,including 1 491 bp coding re-gion,encoding 496 amino acids;6 low complexity regions and 3 conservative LIM domains are included in MnPXN,forming a branch with Litopenaeus vannamei.It is closely related to insects such as silkworm and Tribolium castane-um,and is farthest related to nematodes.The results of quantitative reverse transcriptase-mediated PCR(qRT-PCR)showed that the expression level of MnPXN was high in hepatopancreas,stomach and gill,but low in heart and mus-cle.The expression level of MnPXN gene in hepatopancreas that after 24 h of treatment with 0.242 7 mg·L-1 CdCl2 was significantly higher than that in control group;After 6 h of 0.020 0 mg·L-1 CdCl2 stress,the expression level of MnPXN gene increased significantly,reaching the maximum at 24 h,and gradually recovered to the initial level at 96 h.Besides,RNA interference could significantly reduce the expression level of MnPXN in hepatopancreas and signif-icantly increase the mortality of M.nipponense,indicating that MnPXN could reduce the toxic effect of cadmium stress on M.nipponense,and its mechanism needs further study.
万方数据
维普
