摘要
为深入了解衢州市农业林业科学研究院收集的辣椒种质资源的遗传多样性,提高种质利用率,为辣椒遗传育种提供理论依据.对193 份辣椒种质的 18 个农艺性状进行遗传多样性分析、相关性分析、主成分分析和聚类分析.结果发现,193 份供试材料的果实表型具有丰富的遗传多样性,遗传多样性指数范围为4.95~5.25,成熟果色遗传多样性指数最大.9 个数量性状(果长、果宽、单果重、心室数、主茎高度、首花节位、现蕾期、开花期、坐果期)的变异系数为23.25%~81.98%,其中单果重变异系数最大.各性状间关系复杂,主成分分析中累积贡献率为96%,18 个性状可简化为 6 个主成分.聚类分析将供试材料划分为 3 个类群,其中大部分青熟果颜色为白色的种质资源集中在Ⅰ类群,该类群种质数量最多,有150 份,Ⅱ类群种质大多数引自美国,Ⅲ类群中种质果形较大.鉴于类群间辣椒种质的遗传背景及其在果形、花期、主茎高等方面存在的差异,可进行类群间杂交,从而提高辣椒育种效率.
Abstract
To clearly understanding the genetic diversity of pepper germplasm resources collected by Quzhou Acade-my of Agricultural and Forestry Sciences,improving the utilization of germplasm,and providing theoretical basis for pepper breeding.Genetic diversity analysis,correlation analysis,principal component analysis and cluster analysis were performed on 18 agronomic traits of 193 pepper germplasm resources.The results showed that the fruit pheno-type of the tested materials exhibited abundant genetic diversity,the genetic diversity index ranged from 4.95 to 5.25,and the mature fruit color had the highest genetic diversity index.The coefficient of variation of 9 quantitative traits(fruit length,fruit width,single fruit weight,carpel number,main stem height,first flower node,squaring stage,flowering stage and fruit setting stage)ranged from 23.25%to 81.98%,and the highest coefficient of varia-tion was observed in single fruit weight.The relationship among the traits was complex,and the cumulative contribu-tion rate in principal component analysis was 96%.The 18 traits could be simplified into 6 principal components.The tested materials were divided into 3 groups by cluster analysis,of which groups I contained most of germplasm resources with white green ripe fruit color,the number of germplasm resources in group I was the largest,with 150.Most of the germplasm resources in groupⅡwere introduced from the United States,and the germplasm resources in group Ⅲ had larger fruit shape.In view of the genetic background of pepper germplasm among groups and the differ-ences in fruit shape,flowering period and main stem height,hybridization among groups can be carried out to im-prove breeding efficiency of pepper.
基金项目
衢州市科技局项目(2021K15)
衢州市科技局项目(2022K22)
浙江省蔬菜育种专项(2021C02065-1-4)