Cloning and expression analysis of FLS gene of flavonol synthetase in Lilium auratum and L.speciosum var.gloriosoides
The flavonol synthetase gene FLS were cloned from Lilium auratum and L.speciosum var.gloriosoides,re-spectively,and named as LaFLS and LsFLS.The results showed that the LaFLS and LsFLS genes contained 1 035 bp complete open reading frame and encoded 344 amino acids.The amino acid sequences were highly conserved,and both genes had the DIOX-N domain and the 2-ketoglutaric acid and iron(Ⅱ)dependent dioxygenase domain,be-longing to the 2-ketoglutaric acid and iron(Ⅱ)dependent dioxygenase superfamily.Phylogenetic analysis showed that LaFLS and LsFLS were closely related to Tulipa fosteriana,except for the FLS of oriental hybrids Siberia and Sorbonne.Bioinformatics analysis showed that LaFLS and LsFLS were hydrophilic proteins without signal peptide se-quences and transmembrane domains.Subcellular localization results showed that they were mainly located in the cy-toplasm.The results of gene expression analysis showed that LaFLS and LsFLS increased first,then decreased and then increased with the development of flower bud,and the expressions of LaFLS and LsFLS were significantly higher in the colorless region than in the colored region.
万方数据
维普
