Tissue culture and breeding of Pleione formosana Hayata and chemical composition analysis of its pseudobulbs
The aim of this study was to construct the tissue culture and breeding technology system of Pleione formo-sana Hayata,and analyze the chemical composition characteristics of pseudobulbs of P.formosana Hayata.After seed germination,protocorm growth,pseudobulb proliferation,rooting and seedling culture,the plants of P.formo-sana Hayata were obtained.The pseudobulbs of tissue-cultured P.formosana Hayata plants were used as materials.The chemical composition of pseudobulbs was analyzed by non-targeted metabolomics and HPLC fingerprint.After 2 years of breeding,more than 1 000 seedlings with about 0.5 g pseudobulbs were obtained from a single capsule.Through the analysis of non-targeted metabolomics data,1 229 common compounds were identified in different mate-rials,including 236 carboxylic acids and their derivatives,110 benzene and substituted derivatives,106 organic oxy-gen compounds,94 fatty acyl compounds,43 flavonoids,and 42 steroids and steroid derivatives.Among them,the benzyl esters represented by 1,4-bis[4-(glucoseoxy)benzyl]-2-isobutylmalate are orchid-enriched compounds.Meanwhile the content of 1,4-bis[4-(glucoxy)benzyl]-2-isobutylmalate was 0.53%in pseudobulbs detected by HPLC.The plants of P.formosana Hayata can be bred by tissue culture technology.And the pseudobulbs of P.for-mosana Hayata are rich in benzyl ester compounds as well as P.bulbocodioides(Franch.)Rolfe.
万方数据
维普
