The aim of this study was to construct the tissue culture and breeding technology system of Pleione formo-sana Hayata,and analyze the chemical composition characteristics of pseudobulbs of P.formosana Hayata.After seed germination,protocorm growth,pseudobulb proliferation,rooting and seedling culture,the plants of P.formo-sana Hayata were obtained.The pseudobulbs of tissue-cultured P.formosana Hayata plants were used as materials.The chemical composition of pseudobulbs was analyzed by non-targeted metabolomics and HPLC fingerprint.After 2 years of breeding,more than 1 000 seedlings with about 0.5 g pseudobulbs were obtained from a single capsule.Through the analysis of non-targeted metabolomics data,1 229 common compounds were identified in different mate-rials,including 236 carboxylic acids and their derivatives,110 benzene and substituted derivatives,106 organic oxy-gen compounds,94 fatty acyl compounds,43 flavonoids,and 42 steroids and steroid derivatives.Among them,the benzyl esters represented by 1,4-bis[4-(glucoseoxy)benzyl]-2-isobutylmalate are orchid-enriched compounds.Meanwhile the content of 1,4-bis[4-(glucoxy)benzyl]-2-isobutylmalate was 0.53%in pseudobulbs detected by HPLC.The plants of P.formosana Hayata can be bred by tissue culture technology.And the pseudobulbs of P.for-mosana Hayata are rich in benzyl ester compounds as well as P.bulbocodioides(Franch.)Rolfe.
维普
万方数据
