Isolation,identification and biocontrol mechanism of antagonistic fungus against Chinese pepper gummosis
Chinese pepper gummosis caused by Fusarium tricinctum is a serious disease of Zanthoxylum bungeanum in recent years.In order to obtain the excellent antagonistic strain of F.tricinctum,and the endophytic fungi with strong antagonistic effect were screened and isolated by plate confrontation method.A strain with high antagonistic activity was obtained,and its inhibition rate reached 58.85%,which was named HJ-18.Based on morphological and ITS molecular identification,it was preliminarily identified as Schizophyllum commune(GenBank accession number:OP502072).The antifungal spectrum test showed that it had inhibitory effect on 4 plant pathogenic fungi.After treatment with different light,temperature and pH,the antifungal effect was stable,but sensitive to ultraviolet ray.When the volume fraction of culture filtrate was50%,the inhibition rate of mycelium growth of F.tricinctum was the highest,reaching 66.40%.Scanning electron microscope observation showed that after treatment with culture fil-trate,the mycelium of F.tricinctum was wrinkled,and debris was produced on the surface.The results of pot exper-iment showed that the relative prevention effect of strain HJ-18 was52.65%,which was the same as 100-fold dilu-tion of 22.5%picoxystrobin treatment.The above results show that strain HJ-18 is an endophytic fungus with biocon-trol potential and can be used as a biocontrol agent.
Chinese pepper gummosisendophyticantagonistic strainsculture filtratebiocontrol mechanism
万方数据
维普
