This paper focuses on the extraction process conditions of the antidote components of lotus roots and the antidote efficacy of the fudge prepared.The extraction solvents,solid-liquid ratio,extraction time and extraction tem-perature were investigated by single-factor and orthogonal experiments using lotus root as the raw material and the in vitro activation rate of alcohol dehydrogenase(ADH)as the index.The composition of the lotus root extract was ana-lyzed and used as the raw material for the preparation of alcoholic functional gummies.The scavenging rates of DPPH radicals,ABTS+radicals and·OH radicals were determined by adding different concentrations of lotus root extracts to the gel fudge.The results showed that the best extraction process of lotus root extract was 100%methanol,1∶40 ratio,60℃and 5 h.The in vitro activation rate of ADH was(19.60±2.10)%,and 433 components were ana-lyzed by UPLC-QTOF-MS/MS,the main substances are phenolic acids and flavonoids.The IC50 of lotus root gummy extract for the scavenging of ABTS+ radical,·OH radical and DPPH radical were 14.59 mg·mL-1,101.96 mg·mL-1 and 5.54 mg·mL-1,respectively.Animal experiments demonstrated that lotus root relieving alcoholism func-tional gel fudge significantly increased(P<0.05)and decreased(P<0.05)the liver ADH activity in mice after al-cohol consumption.It also prolonged the mean latency time of intoxication and shortened the recovery time of the re-flex after intoxication.The results of this study suggest that lotus root relieving alcoholism functional gel fudge have certain antidrinking effects.The results of this study provide a new theoretical and practical basis for the development of functional food with the effect of alcohol detoxification and liver protection.
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