Preparation and efficacy evaluation of lotus root relieving alcoholism functional gel fudge
This paper focuses on the extraction process conditions of the antidote components of lotus roots and the antidote efficacy of the fudge prepared.The extraction solvents,solid-liquid ratio,extraction time and extraction tem-perature were investigated by single-factor and orthogonal experiments using lotus root as the raw material and the in vitro activation rate of alcohol dehydrogenase(ADH)as the index.The composition of the lotus root extract was ana-lyzed and used as the raw material for the preparation of alcoholic functional gummies.The scavenging rates of DPPH radicals,ABTS+radicals and·OH radicals were determined by adding different concentrations of lotus root extracts to the gel fudge.The results showed that the best extraction process of lotus root extract was 100%methanol,1∶40 ratio,60℃and 5 h.The in vitro activation rate of ADH was(19.60±2.10)%,and 433 components were ana-lyzed by UPLC-QTOF-MS/MS,the main substances are phenolic acids and flavonoids.The IC50 of lotus root gummy extract for the scavenging of ABTS+ radical,·OH radical and DPPH radical were 14.59 mg·mL-1,101.96 mg·mL-1 and 5.54 mg·mL-1,respectively.Animal experiments demonstrated that lotus root relieving alcoholism func-tional gel fudge significantly increased(P<0.05)and decreased(P<0.05)the liver ADH activity in mice after al-cohol consumption.It also prolonged the mean latency time of intoxication and shortened the recovery time of the re-flex after intoxication.The results of this study suggest that lotus root relieving alcoholism functional gel fudge have certain antidrinking effects.The results of this study provide a new theoretical and practical basis for the development of functional food with the effect of alcohol detoxification and liver protection.
万方数据
维普
