摘要
为探究施用侧孢短芽孢杆菌(Brevibacillus laterosporus)对烤后烟叶钾含量及烟株生理特征的影响,在烟苗移栽期设置不施用侧孢短芽孢杆菌(T1)、侧孢短芽孢杆菌浸根(T2)、侧孢短芽孢杆菌灌根(T3)及侧孢短芽孢杆菌浸根+灌根(T4)4 个处理,测定烤后烟叶钾含量、叶片光合色素含量、抗氧化酶活性及抗病性等指标的变化.结果表明:侧孢短芽孢杆菌的最佳施用方式为浸根+灌根,该处理烤后上部及中部叶的钾含量较T1 处理分别显著(P＜0.05)提高29.86%、67.36%,根系钾含量显著提高 16.58%～28.85%,烟株气候斑病、炭疽病、赤星率、叶斑病的发病率和病情指数显著降低,烟叶的叶绿素、类胡萝卜素含量,及超氧化物歧化酶、过氧化物酶活性分别显著提高 13.21%～18.81%、21.01%～35.41%、9.63%～18.31%、11.62%～13.81%,叶片丙二醛含量显著降低 20.76%～30.99%.综上,在烟苗移栽期以浸根+灌根方式施用侧孢短芽孢杆菌可增强烟株的抗性,提高烤后烟叶的钾含量.
Abstract
To explore the effect of application of Brevibacillus laterosporus on potassium content of cured tobacco leav-es and physiological characteristics of tobacco plants,four treatments were set up in the transplanting period of tobac-co,including no application of B.laterosporus(T1),root soaking of B.laterosporus(T2),root irrigation of B.lat-erosporus(T3)and root soaking +root irrigation of B.laterosporus(T4).The changes of potassium content of cured tobacco leaves,and photosynthetic pigment content,antioxidant enzymes activities,disease resistance of tobacco plants were measured.It was shown that the best application mode of B.laterosporus was root soaking + root irriga-tion.Compared with the T1 treatment,T4 treatment significantly(P＜0.05)increased the potassium content in the upper and middle cured tobacco leaves by 29.86%and 67.36%,respectively.The potassium content in tobacco root was significantly increased by 16.58%-28.85%under T4 treatment,and the incidence and disease index of tobacco weather fleck,tobacco anthracnose,tobacco brown spot and tobacco leaf spot were significanty decreased as compared with the T1 treatments.Besides,the chlorophyll content,carotenoid content,superoxide dismutase acitivi-ty,peroxidase activity were significantly increased by 13.21%-18.81%,21.01%-35.41%,9.63%-18.31%,11.62%-13.81%,respectively,and the malondialdehyde content was significantly decreased by 20.76%-30.99%under T4 treatment than those under T1 treatment.In general,application of B.laterosporu in the transplanting period by root soaking +root irrigation could enhance the resistance of tobacco plants and increase the potaasium content in cured tobacco leaves.
基金项目
中国烟草总公司重庆公司科技项目(A20201NY01-1301)
中国烟草总公司重庆公司科技项目(A20201NY01-1306)
维普
万方数据