TCPs transcription factor regulates plant organogenesis and morphological configuration mainly through regulating cell proliferation and plant hormone pathways,so as to explore the sequence characteristics and expression characteristics of ZjTCP4 transcription factor of Chinese jujube,in order to provide reference for the mechanism study of TCP transcription factor response to drought stress,and lay a foundation for the subsequent molecular breeding of jujube tree.The novel-miR16 secondary structure has the typical hairpin structure of miRNA and is a member of the jujube miR319 family.Most of the degradation sites of target gene ZjTCP4 were located at the 10th and 11th nucleo-tides of the novel-miR16 binding sites.Bioinformatics analysis showed that the full-length cDNA sequence of ZjTCP4 gene was 1 386 bp,predicted to encode 452 amino acid polypeptide,isoelectric point(pI)of 6.51,molecular weight of 49 091.55 u.It should be an intramembranous or extracellular protein,a non-secretory protein,a hydro-philic protein,located in the nucleus.The amino acid sequence encoded by ZjTCP4 gene of Chinese jujube is most similar to AtTCP4 of Arabidopsis thaliana,and has high similarity to AtTCP17,AtTCP18 and AtTCP12 of Arabidopsis thaliana.Novel-miR16 was up-regulated in response to drought stress,while its target gene ZjTCP4 was down-regu-lated under drought stress.Novel-miR16 can regulate ZjTCP4 transcription factor in response to drought stress.Un-der drought stress,the up-regulated expression of novel-miR16 leads to down-regulated expression of target gene ZjTCP4(CCG006181)to improve drought tolerance of Chinese jujube.
关键词
枣树/微RNA/TCP转录因子/生物信息学/亚细胞定位
Key words
Chinese jujube/microRNA/TCP transcription factor/bioinformatics/subcellular localization
维普
万方数据