Transcriptomic analysis and simple sequence repeat markers development of Paeonia suffruti-cosa L.in responses to waterlogging stress
Waterlogging stress is one of the most severe abiotic stress that limits cultivational expansion,fast growth,and high yield of P.suffruticosa.In order to elucidate the mechanism of P.suffruticosa during waterlogging stress,transcriptomic analysis was performed and SSR markers were developed in this study.Six cDNA libraries constructed from mRNAs of waterlogging-treated seedlings and control were sequenced by using the Illumina sequencing platform.Totally,73 925 unigenes were obtained by denovo assembly,creating an initial reference transcriptome dataset of P.suffruticosa.Among them,780 were identified as response genes in early stage of waterlogging including 155 up-reg-ulated genes and 625 down-regulated genes.Functional analysis showed that modifications in the expression of genes involved in signal regulation by transcription factors,DNA replication,ribosome and pyrimidine metabolism might play important roles in responses to waterlogging stress in P.suffruticosa.Based on these high throughput transcrip-tome sequencing data,5 204 SSR markers were excavated.Among these markers,dinucleotide repeats were 58.13%,trinucleotide repeats were 27.11%,and tetranucleotide repeats were 7.24%.110 pairs of SSR marker primers were found in the unigenes of functional annotation related to the target genes of waterlogging stress traits of peony,and 45 pairs of primers were screened,of which 12 pairs could amplify clear target bands,and the polymor-phic SSR marker primers accounted for 26.67%of the total primers.This study provides the first large-scale tran-scriptome annotation of P.suffruticosa which may beneficially elucidate the waterlogging response mechanisms.Be-sides,it provides a valuable genomic resource and a scientific basis for molecular marker assisted selection breeding in waterlogging-tolerant species of P.suffruticosa.
万方数据
维普
