Rice blast disease caused by Magnaporthe oryzae has posed a serious threat to rice yield in China.Stud-ying the gene functions of M.oryzae is beneficial for better understanding the pathogenic mechanism of M.oryzae and lays a foundation for the control of rice blast disease.In this study,a yeast ZDS1 homolog gene MoZDS1 was screened through genetic methods in M.oryzae.The gene deletion mutant ΔMozds1 was successfully obtained by gene knockout method,and it was found that the deletion of MoZDS1 seriously affected the vegetative growth.In or-der to explore the functions of MoZds1,this study analyzed the roles of Mozds1 in maintaining vegetative growth,conidiation,response to cell wall stress and autophagy.The results showed that MoZds1 positively regulated the my-celium growth and negatively regulated the conidia production.In addition,MoZds1 was also involved in the cell wall integrity pathway and regulated the glycogen degradation in conidia,however,the deletion of MoZds1 did not show a significant effect on the autophagy pathway.Overall,this analysis of the function of MoZds1 systematically in M.oryzae,may provide a theoretical basis for further in-depth research on the function of MoZds1.
维普
万方数据