Establishment of sweet potato stem rot resistance identification technology and analysis of germplasm resource resistance
In order to optimize the identification technique for stem rot resistance in sweet potato germplasm,this study analyzed the effects of different inoculation methods and bacterial liquid concentrations on the resistance of sweet potato germplasm.The aim was to establish a standardized identification criterion for stem rot resistance and lay a foundation for disease-resistant breeding in sweet potato.Using the inoculation method of scratching the surface of tissue-cultured sweet potato plantlets and the optimal bacterial liquid concentration(1.1×107 CFU·mL-1),six rep-resentative sweet potato varieties were inoculated and identified.The results of indoor resistance identification were val-idated against field resistance performance and showed a high degree of consistency,indicating the accuracy and relia-bility of the newly developed identification technique.Furthermore,65 sweet potato materials were identified for resist-ance to stem rot.Most of the materials were susceptible or moderately susceptible.However,seven germplasm re-sources(YD7002,DY7082-1,Zhezi No.1,TF1118,DY7032,Nanshu88 and Zhezi No.4)exhibited stable resistance against stem rot in sweet potato.This study established a new identification technique for stem rot resistance in sweet potato and identified germplasm resources with stable resistance,providing important genetic materials for disease-re-sistant breeding.These findings are of significant importance for the prevention and control of sweet potato diseases.
万方数据
维普
