Effect of vibration stress on anthocyanin metabolism and related gene expression in blueberry
Blueberry is rich in bioactive substances such as anthocyanin and has high nutritional value.However,lo-gistics vibration damage can accelerate the loss of nutrients such as blueberry anthocyanin and reduce nutritional quality.This article took the blueberry variety Lanmeiren as the experimental material and studied the effect of vibra-tion stress on the anthocyanin components,metabolic enzymes activities,and genes expression of blueberry by simu-lating logistics vibration.The research results showed that blueberry contained 10 types of anthocyanin monomers,a-mong which the content of quercetin-3-arabinose was the highest.In the early stage of blueberry storage,vibration stress accelerated the accumulation of anthocyanin,significantly increased the activities of phenylalanine ammonia lyase(PAL)and flavonoid glycosyltransferase(UFGT),and induced the expression of genes related to anthocyanin synthesis,such as VcPAL1,VcDFR2,VcCHI1,and VcUFGT.In the later stage of storage,the activities of PAL,chalcone isomerase(CHI),dihydroflavonol reductase(DFR),and UFGT in the vibration stress group were signifi-cantly lower than those in the control group.Meanwhile,vibration stress also delayed the decrease in the activity of enzymes related to anthocyanin degradation,such as peroxidase(POD),polyphenol oxidase(PPO)and anthocya-nin-β-glucosidase,inhibited the expression of genes related to anthocyanin synthesis,and promoted the expression of genes related to anthocyanin degradation,such as VcPOD1,VcPOD2,VcPOD3,and VcPPO1.In summary,vibra-tion stress accelerated the accumulation of anthocyanin in blueberry during early storage by increasing the activity of anthocyanin synthase and the expression of related enzyme genes.In the later stage of storage,vibration stress pro-moted the degradation of anthocyanin by delaying the decrease of anthocyanin degrading enzymes activities and relat-ed enzyme genes expression.The research results provided a theoretical basis for regulating the metabolism of blue-berry anthocyanin.
万方数据
维普
