Identification and expression analysis of Thionin-like gene family in wheat
Thionin-like(Thil)has important biological functions in plant defense response to pathogenic microorgan-isms.In this study,the Thil gene family of Triticum aestivum L.was initially identified using the homologous search and bioinformatics method based on Thil genes of the model plant Arabidopsis thaliana.A total of 26 Thil genes are included in the wheat genome.Structural analysis of gene sequences showed that the conserved motif structure exists in the Thil family,Thil genes encode proteins of 79-196 amino acids,most proteins have less than 140 amino acids,and 20 Thil proteins contain signal peptides.The cis-acting element prediction indicated that promoter ele-ments of the Thil genes are mainly affected by corresponding hormones about adversity stress such as abscisic acid.Gene expression profiles showed that diversifying expression of the Thil family genes exists in response to pathogenic infection.qRT-PCR analysis showed that the expressions of TaThil-4A,TaThil-4B,and TaThil-4D were induced in the early response to wheat leaf rust fungus.The results of this study can provide a reference for wheat disease resist-ance breeding in wheat and functional study and application of the Thil gene family.
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