Comprehensive evaluation of agronomic characteristics of recombinant inbred lines of Tarta-ry buckwheat based on principal component analysis
In order to screen accessions with better comprehensive agronomic traits and materials for breeding high yield Tartary buckwheat varieties,ten agronomic traits of 58 recombinant inbred lines(RILs)with high yield poten-tial and the two parents were evaluated by genetic variation analysis,correlation analysis,principal component analy-sis and cluster analysis.The results showed that the coefficient of variation ranged from 5.71％to 31.55％for the 10 agronomic traits of 58 RILs.The coefficient of variation of seed yield,growth period and branch number of main stem were larger,and the coefficient of variation of seed width and seed perimeter were smaller.The seed yield was signif-icantly positively correlated with seed area,seed perimeter,plant height and 1 000-seed weight at P＜0.01 level,and significantly positively correlated with seed length and seed width at P＜0.05 level.There was a significant neg-ative correlation between seed yield and growth period at P＜0.01 level.The absolute value of correlation coeffecient of the above indexes decreased as follows:1 000-grain weight＞plant height＞seed area＞growth period＞seed per-imeter＞seed width＞seed length.The results of principal component analysis showed that the cumulative contribu-tion rate of the top four principal components was 86.987％,and the four principal components were seed shape and yield factor(39.940％),seed width factor(24.478％),plant height factor(11.667％),and branch number of main stem and growth period factor(10.893％).Based on the comprehensive evaluation results and analysis of vari-ance between RILs and parents,seven excellent non-rice RILs were screened out,namely,R64,R103,R164,R84,R192,R153 and R214.The seven RILs were grouped into the C2 group with high yield,large seed,high stem and short growth period in cluster analysis,which could be used as promotion varieties for demonstration and high quality germplasm resources of conventional Tartary buckwheat breeding in southwest China.The yield of three rice type RILs,namely,R52,R198 and R101,was significantly higher than that of the parent Xiaomiqiao at P＜0.01 or P＜0.05 level,which could be used as materials for the breeding of high-yield and thin-shell Tartary buckwheat.
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