Gut microbial profiles and its developmental changes of grass carp(Ctenopharyngodon idella)
The study was aim to expolar the diversity of the microbial structure in different intestinal segments during the growth and development processes of grass carp.The experiment was conducted in two parts,Part Ⅰ:Eight grass carp at 24 months were anesthetized with MS-222 for DNA extraction and 16S rRNA sequencing analysis;Part Ⅱ:25 grass carp with different ages,including 1 month,7 months,15 months and 24 months,were purchased from a typical Kaihua clear water fish breeding base in Kaihua County,Quzhou City,Zhejiang Province.To ensure suffi-cient sample size and minimize individual differences,the contents of the hindgut segments of each five fish were mixed into a single sample.The contents of grass carp at different months were extracted for DNA extraction and 16S rRNA sequencing analysis.The results indicated that:1)Alpha diversity analysis showed that the diversity and rich-ness of microbial community in hindgut were significantly higher than those in foregut and midgut(P＜0.05);Beta diversity analysis showed that the microbial communities in foregut and midgut were similar,but significantly different from those in hindgut(P＜0.05).2)LEfSE analysis showed that Streptococcus and Ralstonia were dominant bacteri-a in the foregut.Escherichia-Shigella and Aliihoeflea were dominant bacteria in the midgut segment.Leptolyngbya and Cetobacterium are the main dominant genera in the hindgut.3)The diversity and richness of microbial communi-ty in the hindgut of grass carp significantly decreased from 1 month to 24 months of age(P＜0.05);Beta diversity analysis showed that there were significant differences in the microbial structure of the hindgut of spring water grass carp at different months(P＜0.05).4)Heatmap shows that Rubellimicrobium and Synechococcus are the main dom-inant bacteria in the hindgut at the age of 1 month.Aestuariimicrobium and Streptomyces were the dominant genera in the hindgut intestine of the 7-month-old.Kineosporia and Prevotella were dominant genera in the 15-month-old hind-gut,while Vibrio and Mesonia were dominant genera in the 24-month-old hindgut.This indicates that there are differ-ences in the composition and structure of microbia communities in different intestinal segments of grass carp,as well as developmental changes in the structure of gut microbiota during different stages of growth.
grass carpmicrobial community structuredevelopmental change
NETL
NSTL
万方数据
