大麦籽粒相关性状全基因组关联分析
Genome-wide association analysis of barley grain related traits
栾海业 1朱琳洁 1李钰 1孟炜 1刘雨倩 1徐肖 2刘方方 1沈会权2
作者信息
- 1. 盐城师范学院,江苏盐城 224002
- 2. 江苏沿海地区农业科学研究所,江苏盐城 224002
- 折叠
摘要
大麦籽粒形状和千粒重是决定大麦产量及用途的主要因素,挖掘相关候选基因对于提高大麦产量和品质具有重要意义.以国内外具有代表性的242份大麦自然群体为研究对象,对粒长、粒宽和千粒重进行全基因组关联分析.研究结果表明,大麦籽粒大小和千粒重表型变异丰富,变异系数范围为6.65％～19.98％.粒长、粒宽与千粒重均呈极显著正相关,且千粒重与粒宽的相关性高于粒长的.3个性状共检测到52个相关的SNP位点,在7条染色体均有分布.通过比对分析,筛选了 6个相关性状的候选基因,在7号染色体上的乙烯响应因子与粒宽、千粒重均相关,表明该基因存在一因多效的功能.研究结果为大麦产量、品质的遗传改良提供了基因资源和理论基础.
Abstract
Grain size and thousand grain weight is the main factors that determine the barley yield and uses.It is of great importance to mine candidate genes related to grain for improving the yield and quality of barley.In this study,genome-wide association study(GWAS)was carried out to detect the candidate genes associated with grain traits in natural population containing 242 barley germplasm resources.The results showed that these traits showed rich phe-notypic variation,with the coefficient of variation ranged from 6.65％-19.98％.Thousand grain weight was signifi-cantly correlated with grain length and grain width.The correlation coefficient between thousand grain weight and grain width was appreciably higher than thousand grain weight and grain length.Fifty-two SNP locus were detected by GWAS on 7 chromosomes.BLAST analysis showed that six candidate genes related to grain traits were selected.The ethylene response factor on chromosome 7 was simultaneously correlated with grain width and thousand grain weight,indicating that there was a multi-effect function of this gene.The results of this study provide new genetic resources and theoretical basis for the genetic improvement of barley yield and quality.
关键词
大麦/籽粒相关性状/全基因组关联分析/候选基因Key words
barley/grain trait/genome-wide association analysis/candidate gene引用本文复制引用
基金项目
国家大麦青稞产业技术体系建设项目(CARS-05)
江苏省自然科学基金(BK20201215)
盐城市农业重点研发计划(YCBN202308)
出版年
2024
NETL
NSTL
万方数据