家蚕素斑品种菁松的茶斑限性定向转育
The directional transfer of the plain silkworm variety Jingsong into a sex-limited multilunar silkworm variety
廖鹏飞 1李琼艳 1罗顺高 1刘敏 1朱红涛 1李继娅 1白红英 1陈海佺 1范永慧 1董占鹏1
作者信息
- 1. 云南省农业科学院蚕桑蜜蜂研究所,云南蒙自 661101
- 折叠
摘要
为在家蚕现行素斑品种菁松的幼虫发育早期进行雌雄性别鉴别,实现雌雄分养分缫差异化利用目的,用普斑限性品系云蚕7A的雌与茶斑品系Cb的雄杂交后,再分别与现行素斑品系菁松A、菁松B杂交,其后代连续数次回交素斑亲本后自交,结合分类选择进行个体和蛾区选择淘汰,将云蚕7A的普斑限性基因和Cb的茶斑基因转入现行素斑品种菁松中,定向转育得到菁A茶限和菁B茶限.结果表明:转育过程中,幼虫斑纹分化成颜色与形状易于分辨的茶斑与非茶斑2种类型,转育得到的菁A茶限和菁B茶限幼虫在3龄初期就会呈现茶斑颜色深浅明显不同的分化,能准确分辨雌雄,其虫蛹率分别为96.91％和96.40％,全茧量分别为1.62 g和1.56 g,茧层率分别为23.78％和23.26％,经济性状与其轮回亲本的差异较小,表现出健康好养、茧质优良等特性.研究结果可为创建特殊性状的育种材料、差异化利用雌雄蚕,以及降低雄蚕种繁育成本奠定基础.
Abstract
To identify the gender of the larvae in the early developmental stages of the current plain silkworm variety Jingsong,for the purpose of achieving sex-separation in rearing and differential utilization,the female of the sex-lim-ited normal marking silkworm race Yuncan 7A was crossed with the male of multilunar silkworm race Cb.Then,these hybrids were respectively crossed with the commercial plain silkworm race Jingsong A and Jingsong B.The progeny were backcrossed to the commercial plain silkworm race for several times and then selfed,combined with categorized selection for individual and batches.This process introduced sex-limited normal marking gene of Yuncan 7A and multilunar gene of Cb into the commercial plain silkworm race Jingsong,directed breeding resulted in Jing A Chaxian and Jing B Chaxian.The results showed that during the breeding process,the larval spot differentiation turned into easy-to-distinguish multilunar and non-multilunar types by color and shape.The cultivated Jing A Chax-ian and Jing B Chaxian larvae displayed clearly different color differentiation of multilunar at the beginning of the 3rd instar,allowing for accurate gender identification.The economic traits of Jing A Chaxian and Jing B Chaxian,such as the larva-pupa rate of 96.91％and 96.40％,the whole cocoon weight of 1.62 g and 1.56 g,the cocoon shell ra-tio of 23.78％and 23.26％,were slightly different from their recurrent parents,exhibiting characteristics of good health,easy rearing,and high-quality cocoons.This study laid a foundation for creating breeding materials with spe-cial traits,the differential utilization of male and female silkworms,and reducing the breeding costs of male silk-worms.
关键词
家蚕品种/普斑限性/素斑/茶斑限性/定向转育Key words
silkworm race/sex-limited normal marking/plain/sex-limited multilunar/directional transfer引用本文复制引用
基金项目
向仲怀院士工作站项目(202305AF150166)
云南省科技厅重点研发(农业领域)专项(202102AE090010)
云南省现代农业蚕桑产业技术体系建设项目(2019KJTX006)
现代农业产业技术体系建设专项(CARS-18)
出版年
2024
NETL
NSTL
万方数据