为了挖掘与萝卜表型性状显著相关的分子标记,对60份萝卜的14个表型数据进行鉴定,筛选出具有多态性的分子标记29个(其中12个SNP标记和17个InDel标记)进行遗传多样性分析,并利用TAS-SEL5.0软件的广义线性模型(general linear mode,GLM)对萝卜的14个表型数据进行关联分析.研究结果表明,29个分子标记的等位基因(Na)数范围在2～3个,平均为2.14个;主等位基因频率(MAF)为0.53-0.94个,平均为0.68个;每个标记的期望杂合度(He)范围为0.12-0.93,平均为0.63.每个位点的多态性信息含量(PIC)值范围在0.11-0.37,平均为0.32;在12个表型性状中关联到17个显著相关的分子标记位点(其中7个SNP标记和10个InDel标记)(P≤0.01),贡献率在7.24％-23.25％.
Development of SNP and InDel markers in radish and their association with phenotypic char-acters
In order to explore the molecular markers associated with significant phenotypic traits of radish,we identi-fied 14 phenotypic data of 60 radish samples,and screened out 29 molecular markers with polymorphism(12 SNP markers and 17 InDel markers)for genetic diversity analysis.The general linear mode(GLM)of TASSEL5.0 soft-ware was used for correlation analysis of 14 phenotypic data of radish.The results showed that the number of alleles(Na)of 29 molecular markers ranged from 2 to 3,with an average of 2.14.The frequency of major alleles(MAF)ranged from 0.53 to 0.94,with an average of 0.68.The expected heterozygosity(He)for each marker ranged from 0.12 to 0.93,with an average of 0.63.The polymorphism information content(PIC)values of each locus ranged from 0.11 to 0.37,with an average value of 0.32.Among the 12 phenotypic traits,17 significantly correlated mo-lecular marker sites(7 SNP markers and 10 InDel markers)were associated(P≤O.01),and the contribution rate was 7.24％-23.25％.
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