首页|两种树龄腾冲红花油茶花梗内生真菌的多样性

两种树龄腾冲红花油茶花梗内生真菌的多样性

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以腾冲红花油茶花梗为试材,采用高通量测序的方法,研究了 12年生(N组)和60年生(O组)树龄的腾冲红花油茶花梗内生真菌群落结构及其多样性.结果表明,两种树龄腾冲红花油茶花梗内生真菌OTU和Alpha分析结果存在差异,物种多样性和丰富度差异显著.在两种树龄腾冲红花油茶花梗中子囊菌门和担子菌门是优势菌门(二者丰度之和>90%),但目、科水平上两组样品优势种群差异巨大,很多物种(真菌)只能分类到目水平,其中O组的煤炱目未分类科和N组银耳目未分类科占比最大.内生真菌功能预测得到11个生态共位群,未知(Unknown)占比最高,动物病原菌、植物病原菌、真菌寄生菌等与各种腐生菌组成了多功能的营养型,内生真菌中病原菌、寄生菌和腐生菌的丰度受树龄影响较大.综上所述,树龄大的腾冲红花油茶花梗中内生真菌多样性和丰富度显著增多,并且优势真菌种类、丰度和功能也发生改变,因此,利用内生真菌时树龄因素不可忽视.
Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate
Taking the pedicel of Camellia reticulata as the test material,the community structure and diversity of en-dophytic fungi in the pedicel of Camellia reticulata at 12 and 60 years old were studied by high-throughput sequen-cing.The analysis results showed that there were differences in OTU and Alpha results of endophytic fungi in the pedicels of Camellia reticulata of two tree ages,and there were significant differences in species diversity and rich-ness.Ascomycota and Basidiomycota were the dominant phyla(the sum of their abundance>90%)in the pedicels of Camellia reticulata of two tree ages,but the dominant populations of the two groups of samples at the level of order and family were very different.Many species(fungi)can only be classified to the level of order,among which un-classified_o_Capnodiales of the O group and unclassified_o_Tremellales of the N group accounted for a large propor-tion.The function of endophytic fungi was predicted to obtain 11 ecological symbionts,with the highest proportion of unknown.Animal pathogens,plant pathogens,fungal parasites,and various saprophytic bacteria formed a multifunc-tional nutrient type.The abundance of pathogens,parasites,and saprophytic bacteria in endophytic fungi was greatly affected by tree age.In summary,the diversity and richness of endophytic fungi in the pedicels of Camellia reticulata increased significantly,and the species,abundance,and function of dominant fungi also changed.Therefore,the age factor cannot be ignored when using endophytic fungi.

Camellia reticulatetree agediversity of endophytic fungihigh-throughput sequencing

黄融、孟庆鑫、吴晓漫、荀利杰、张俊丽、董霞、董坤、龚雪阳

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云南农业大学动物科学技术学院/东方蜜蜂研究所/云南省蜜蜂资源可持续利用工程研究中心,云南昆明 650201

云南农业大学食品科技学院,云南昆明 650201

腾冲红花油茶 树龄 内生真菌多样性 高通量测序

国家自然科学基金现代农业产业技术体系建设项目蜜蜂项目云南省教育厅科研项目云南农业大学科研启动基金

32060241CARS-44-KXJ132023Y0980KY2018-37

2024

浙江农业学报
浙江省农业科学院 浙江省农学会

浙江农业学报

CSTPCD北大核心
影响因子:0.765
ISSN:1004-1524
年,卷(期):2024.36(5)
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