Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate
Taking the pedicel of Camellia reticulata as the test material,the community structure and diversity of en-dophytic fungi in the pedicel of Camellia reticulata at 12 and 60 years old were studied by high-throughput sequen-cing.The analysis results showed that there were differences in OTU and Alpha results of endophytic fungi in the pedicels of Camellia reticulata of two tree ages,and there were significant differences in species diversity and rich-ness.Ascomycota and Basidiomycota were the dominant phyla(the sum of their abundance>90%)in the pedicels of Camellia reticulata of two tree ages,but the dominant populations of the two groups of samples at the level of order and family were very different.Many species(fungi)can only be classified to the level of order,among which un-classified_o_Capnodiales of the O group and unclassified_o_Tremellales of the N group accounted for a large propor-tion.The function of endophytic fungi was predicted to obtain 11 ecological symbionts,with the highest proportion of unknown.Animal pathogens,plant pathogens,fungal parasites,and various saprophytic bacteria formed a multifunc-tional nutrient type.The abundance of pathogens,parasites,and saprophytic bacteria in endophytic fungi was greatly affected by tree age.In summary,the diversity and richness of endophytic fungi in the pedicels of Camellia reticulata increased significantly,and the species,abundance,and function of dominant fungi also changed.Therefore,the age factor cannot be ignored when using endophytic fungi.
Camellia reticulatetree agediversity of endophytic fungihigh-throughput sequencing