Pathogen identification of root rot of Curcuma wenyujin
At main production areas of Curcuma wenyujin in Taoshan Town,Rui'an City,Zhejiang Province,large-scale lodging of C.wenyujin and severe rot of their underground tubers occurred in some fields.To identify the patho-gen that causes the disease,the bacteria strain JF1 was isolated from the rotten tuber of C.wenyujin by using LB and TTC(2,3,5-triphenyltetrazolium chloride)medium.The pathogenicity of isolated strain JF1 was confirmed by using three inoculation methods,including root irrigation,bud infection and rhizome infection.The taxonomic status of pathogenic bacteria was identified according to the morphological characteristics,physiological and biochemical tests,phylogenetic analysis of multiple gene sequences(16S rDNA,rpoB,gyrB).The results showed that the pathogenic bacteria causing root rot of C.wenyujin was Enterobacter mori.
Curcuma wenyujinroot rotpathogen identificationEnterobacter mori