Preliminary study on weeding mechanism of Alternaria gaisen GD-011 strain
Recently,microbial herbicides have become a research hotspot due to their advantages in safety and envi-ronment protection.In order to explore the weeding mechanism of Alternaria gaisen GD-011 strain,experiments were carried out on Elsholtzia densa Benth by observing the ultrastructure changes of E.densa leaves after inoculation of GD-011 strain,as well as determining the changes of physiological and biochemical indexes of leaves.The results showed that the mycelia of strain GD-011 invaded E.densa through stomata to leaf tissue,parasitically reproduced in the tissue,produced spores,and gradually destroyed the tissue.After inoculation of strain GD-011,the malondialde-hyde(MDA)content in the leaves of E.densa gradually increased.Meanwhile,the activities of antioxidant enzyme systems,such as superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD),increased first and then decreased.The soluble protein content in the leaves of E.densa after inoculation for 2-7 d was significantly(P<0.05)higher than that in the control.In general,it was inferred that the GD-011 strain achieved weeding effect by in-fecting through stomata,destroying the cell membrane and tissue structure and inhibiting self-repair,etc.
microbial herbicidesphysiology and biochemistryultrastructureweeding mechanism