Study on the extraction processing and antioxidant activities of saponins from Polygonatum cyrtonema Hua roots
The roots of Polygonatum cyrtonema Hua have long been considered as by-product with low value,and few relevant studies have been reported.This study aims to clarify the desirable extraction process of saponins from the roots of Polygonatum cyrtonema Hua and evaluate the antioxidant activities of the obtained saponins.Response sur-face analysis presented that the optimum extraction method was 72%ethanol,1∶25 g·mL-1,and 41 min.The rela-tionship between Y(extraction rate)and factor A(ethanol volume fraction),factor B(solid-liquid ratio),factor C(extraction time)was presented as Y=5.58+0.516 2A+0.078 8B+0.05C-0.02AB-0.007 5AC-0.002 5BC-1.38A2-0.439 5B2-0.257C2.The peak of saponins extraction was 5.62%,which is very close to the estimated peak(5.628%).Antioxidant test exhibited that saponins have good antioxidant activities with 0.87 mg·mL-1 IC50DPPH and 0.68 mg·mL-1 1C50ABTS.Overall,the saponins in the roots of Polygonatum cyrtonema Hua have consid-erable potential in use.This study can provide an important reference basis for the comprehensive utilization of Po-lygonatum cyrtonema Hua,as well as other by-products.