Identification and expression analysis under saline-alkali stress of ZF-HD gene family in sor-ghum
ZF-HD(zinc finger-homeodomain),a transcription factor family only in plants,plays important role in plant growth and development and stress response.In order to analyze the composition and potential biological func-tions of ZF-HD family genes in sorghum genome(SbZF-HD),the SbZF-HD genes and their encoded amino acid se-quence were analyzed by bioinformatics analysis combined with sorghum transcriptome data.The results showed that a total of 14 SbZF-HD genes were unevenly distributed on 8 chromosomes of sorghum,and the gene structures were different.The motif composition of the deduced amino acids was similar,and the advanced structure of the protein was dominated by random coils.The phylogenetic tree analysis of ZF-HD protein showed that SbZF-HD was closely related to ZF-HD of maize and far from ZF-HD of Arabidopsis thaliana.The SbZF-HD promoter region contains mul-tiple hormone regulatory elements,stress response elements and light response elements.Tissue expression analysis showed that SbZF-HD genes had tissue expression specificity,and the expression level were higher in root,leaf and seed.Transcriptome analysis showed that SbZF-HD2,SbZF-HD7,SbZF-HD8 and SbZF-HD12 genes showed oppo-site expression trends in two brewing sorghum materials with different saline-alkali tolerance under saline-alkali stress,which might play an important role in the saline-alkali tolerance mechanism of sorghum.In summary,the SbZF-HD family members might be the key genes under the regulation of saline-alkali stress in sorghum.This study provided genetic resources for sorghum saline-alkali tolerance transgenic breeding.
NETL
NSTL
万方数据
