高粱ZF-HD基因家族鉴定与盐碱胁迫下的表达分析
Identification and expression analysis under saline-alkali stress of ZF-HD gene family in sor-ghum
吴国江 1周伟 1李艳肖 1侯杰 1杨志强 1周亚星1
作者信息
- 1. 内蒙古民族大学农学院,内蒙古通辽 028000
- 折叠
摘要
ZF-HD(zinc finger-homeodomain)是仅存在于植物中的转录因子家族,在植物的生长发育和逆境应答过程中发挥着重要作用.为分析ZF-HD基因家族成员在高粱基因组中的组成与潜在的生物学功能,利用生物信息学分析方法并结合高粱转录组数据,对高粱ZF-HD基因(SbZF-HD)及其编码氨基酸序列进行分析.结果表明:共有14个SbZF-HD基因不均匀地分布在高粱的8条染色体上,且基因结构各异;它们编码的氨基酸的基序组成较为相似,蛋白质的高级结构均以无规则卷曲为主.ZF-HD蛋白质的进化树分析结果表明,SbZF-HD与玉米的ZF-HD亲缘关系较近,与拟南芥的ZF-HD亲缘关系较远.SbZF-HD启动子区域中包含多个激素调控类元件、胁迫响应类元件和光响应元件.SbZF-HD基因组织表达分析显示,SbZF-HD基因有组织表达特异性,在根、叶和种子中的表达量较高.转录组分析表明,盐碱胁迫条件下,SbZF-HD2、SbZF-HD7、SbZF-HD8和SbZF-HD12这4个基因在2份盐碱耐性不同的酿造高粱材料中表现出相反的表达趋势,可能在高粱耐盐碱机制中发挥着重要作用.综上,SbZF-HD基因家族成员可能是高粱耐盐碱胁迫的关键调控基因,该研究为高粱耐盐碱转基因育种提供了基因资源.
Abstract
ZF-HD(zinc finger-homeodomain),a transcription factor family only in plants,plays important role in plant growth and development and stress response.In order to analyze the composition and potential biological func-tions of ZF-HD family genes in sorghum genome(SbZF-HD),the SbZF-HD genes and their encoded amino acid se-quence were analyzed by bioinformatics analysis combined with sorghum transcriptome data.The results showed that a total of 14 SbZF-HD genes were unevenly distributed on 8 chromosomes of sorghum,and the gene structures were different.The motif composition of the deduced amino acids was similar,and the advanced structure of the protein was dominated by random coils.The phylogenetic tree analysis of ZF-HD protein showed that SbZF-HD was closely related to ZF-HD of maize and far from ZF-HD of Arabidopsis thaliana.The SbZF-HD promoter region contains mul-tiple hormone regulatory elements,stress response elements and light response elements.Tissue expression analysis showed that SbZF-HD genes had tissue expression specificity,and the expression level were higher in root,leaf and seed.Transcriptome analysis showed that SbZF-HD2,SbZF-HD7,SbZF-HD8 and SbZF-HD12 genes showed oppo-site expression trends in two brewing sorghum materials with different saline-alkali tolerance under saline-alkali stress,which might play an important role in the saline-alkali tolerance mechanism of sorghum.In summary,the SbZF-HD family members might be the key genes under the regulation of saline-alkali stress in sorghum.This study provided genetic resources for sorghum saline-alkali tolerance transgenic breeding.
关键词
高粱/ZF-HD基因/表达模式/盐碱胁迫Key words
sorghum/ZF-HD gene/expression pattern/saline-alkali stress引用本文复制引用
基金项目
内蒙古自然科学基金(2023LHMS03018)
科尔沁沙地生态农业国家民委重点实验室开放基金项目(MDK2022023)
内蒙古自治区科技计划(2020GG0102)
内蒙古自治区高等学校青年科技英才(NJYT22052)
2022年度自治区直属高校基本科研业务费项目()
出版年
2024
NETL
NSTL
万方数据