Comparison of purine alkaloids and catechin components of wild Camellia tachangensis in Pu'an County and Panzhou County,Guizhou Province,China
The objective of this study was not only to explore the biochemical differences between wild Camellia tachangensis in Pu'an County and Panzhou County,but also to provide the scientific basis for the protection,develop-ment and utilization of wild C.tachangensis in Guizhou.A total of 40 C.tachangensis germplasm resources from Pu'an County and Panzhou County were used as materials,the contents of theobromine,theophylline,theacrine,caf-feine,gallic acid,epigallocatechin(EGC),catechin(C),epicatechin(EC),epigallocatechin gallate(EGCG),gallocatechin gallate(GCG)and epigallocatechin gallate(ECG)were determined by high performance liquid chro-matography(HPLC).Cluster analysis and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to classify C.tachangensis germplasm resources from two regions and select key differential compounds.The re-sults showed that the main purine alkaloids in C.tachangensis from Pu'an and Panzhou were caffeine and theacrine,respectively.ECG and EC were the prominent components of catechin in C.tachangensis.The contents of theacrine,caffeine,gallic acid,C and EC in C.tachangensis from two regions were significantly different.The contents of caf-feine and gallic acid in C.tachangensis form Pu'an were average to 28.53 mg·g-1 and 0.29 mg·g-1,which were significantly higher than those from Panzhou.The contents of theacrine,C and EC in C.tachangensis from Panzhou were average to 22.88 mg·g-1,2.37 mg·g-1 and 36.83 mg·g-1,which were extremely significantly higher than those from Pu'an.The correlation analysis showed that there were strong correlation between 11 biochemical compo-nents,with 14 pairs showing significant or extremely significant positive correlations,16 pairs showing significant or extremely significant negative correlations.Based on the hierarchical clustering of 11 biochemical components,40 C.tachangensis germplasm resources could be divided into 3 groups.All 30 C.tachangensis germplasm resources from Panzhou were composed group Ⅰ and the main biochemical characteristics were high content of theacrine and C,and low content of caffeine;10 C.tachangensis germplasm resources from Pu'an were separated into 2 categories,GT-PA-03 and other 6 germplasm resources constituted group Ⅱ,the main biochemical characteristics were high content of caffeine,EC,ECG,and low content of theacrine,gallic acid,EGC,C,EGCG;GT-PA-01,GT-PA-02 and GT-PA-09 were composed group Ⅲ and the main biochemical characteristics were high content of gallic acid,EGC and EGCG.OPLS-DA results showed that caffeine,theacrine and gallic acid were the key biochemical factors to distin-guish C.tachangensis germplasm resources from Pu'an and Panzhou.A series of specific genetic resources were screened out from the 40 C.tachangensis germplasm resources,which included three high theacrine(＞25.0 mg·g-1)germplasm resources,two high GCG(＞15.0 mg·g-1)germplasm resources,three high ECG(＞80.0 mg·g-1)germplasm resources,three high EC(＞50.0 mg·g-1)germplasm resources.
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