桑树桑黄JM-1胞外多糖液态培养基优化及其抗氧化性研究
Optimization and antioxidant activity of liquid medium of extracellular polysaccharides from Sanghuangporus sanghuang JM-1
曹乃馨 1罗阳兰 2阎勇 2解修超 3张雯龙2
作者信息
- 1. 广西壮族自治区农业科学院微生物研究所,广西南宁 530007;陕西理工大学生物科学与工程学院,陕西汉中 723000
- 2. 广西壮族自治区农业科学院微生物研究所,广西南宁 530007
- 3. 陕西理工大学生物科学与工程学院,陕西汉中 723000
- 折叠
摘要
为优化桑树桑黄胞外多糖液态发酵培养基配方并探究其体外抗氧化活性,利用组织分离法纯化并鉴定1株桑树桑黄菌株JM-1,通过Box-Behnken响应面法优化菌株胞外多糖液态培养基配方,并考察其体外抗氧化活性.结果表明,优化后的液体培养基配方为玉米粉5.4g·L-1、葡萄糖17.0 g·L-1、蛋白胨5.8g·L-1、维生素 B1(VB1),10.0 mg·L-1、KH2PO4 3.0 g·L-1、MgSO4·7H2O 5.0 g·L-1,该条件下菌株 JM-1 产胞外多糖含量为5.918 g·L-1,比基础培养基提高了 13.63％.抗氧化活性试验结果表明,在质量浓度为5 mg·mL-1时,JM-1菌株产胞外多糖的还原能力为3.187 mmol·L-1,对DPPH自由基和羟自由基的清除率分别为63.9％、54.3％.研究结果可为桑黄资源的开发利用提供科学参考.
Abstract
In order to optimize the liquid fermentation medium formula of Sanghuangporus sanghuang JM-1 extracel-lular polysaccharide and explore its antioxidant activity in vitro,JM-1 was purified and identified by tissue separation method.The liquid medium condition of extracellular polysaccharides from S.sanghuang JM-1 fermentation was opti-mized by Box-Behnken response surface methods.And the antioxidant activity of the produced extracellular polysac-charides was determined in vitro.The results showed that the optimized liquid medium formula was cornmeal 5.4 g·L-1,glucose 17.0 g·L-1,peptone 5.8 g·L-1,vitamin B1(VB1)10.0 mg·L-1,KH2PO4 3.0 g·L-1,MgSO4·7H2O 5.0 g·L-1.Under the experimental conditions,the content of extracellular polysaccharide was 5.918 g·L-1,which was increased by 13.63％than the basal medium.The extracellular polysaccharide produced by JM-1 had the reducing capacity and the scavenging ability of DPPH free radical and hydroxyl radical.At the mass concentration of 5 mg·mL-1,the reduction ability was 3.187 mmol·L-1,and the scavenging rate were 63.9％and 54.3％for DP-PH free radical and hydroxyl radical,respectively.This study provided scientific reference for the development and utilization of Sanghuangporus sp.resources.
关键词
桑黄/胞外多糖/Plackett-Burman设计/Box-Behnken响应面法/抗氧化活性Key words
Sanghuangporus sp./extracellular polysaccharides/Plackett-Burman design/Box-Behnken response surface method/antioxidant activities引用本文复制引用
基金项目
广西农业科学院科技发展基金(桂农科2021JM85)
广西农业科学院科技发展基金(桂农科2022JM07)
创新联盟专项—科技先锋队"强农富民""六个一"专项行动(桂农科盟202308)
出版年
2024
NETL
NSTL
万方数据