CRISP3 gene SNP identification and its impact on reproductive traits in Kela pigs
To analyze the impact of single nucleotide polymorphism(SNP)loci in the Cysteine Rich Secretory Pro-tein 3(CRISP3)gene on reproductive traits of Kele pigs,Sanger sequencing was used to detect SNP mutations in the CRISP3 gene of 250 healthy and prolific Kele sows.SPSS 22.0 software was utilized to analyze the association be-tween SNP loci and six reproductive traits in Kele pigs,including total number of piglets born,primary litter weight,average birth weight,weaning litter weight,number of weaned piglets,and average weaning weight.The result showed that in the CRISP3 gene of Kele pigs,three SNP mutations were identified in the fourth intron(g.43796318 T＞C,g.43796424T＞A,and g.43796425G＞T),each having three genotypes.The dominant genotypes for g.43796318 T＞C and g.43796424 T＞A were TT,with the dominant alleles being T,while the dominant genotype for g.43796425 G＞T was GG,with the dominant allele being G.The x2 test revealed that the genotype distribution of g.43796318T＞C was in Hardy-Weinberg equilibrium(P＞0.05),whereas the genotype distributions of g.43796424T＞A and g.43796425 G＞T significantly(P＜0.01)deviated from Hardy-Weinberg equilibrium.All three SNP loci exhibited moderate polymorphism with polymorphism information content(PIC)ranging from 0.25 to 0.50.Linkage disequilibrium analysis showed strong linkage disequilibrium between g.43796424T＞A and g.43796425G＞T.The association analysis demonstrated that the TT and CC genotypes of g.43796318 T＞C were significantly(P＜0.05)higher in primary litter weight and average birth weight than those of the TC genotype.The TA genotype of g.43796424T＞A had significantly(P＜0.05)higher number of piglets born alive,primary litter weight,and number of weaned piglets compared to the TT and AA genotypes.The GT genotype of g.43796425 G＞T showed significantly(P＜0.05)higher number of piglets born alive,primary litter weight,and number of weaned piglets than those of the GG and TT genotypes.In the diplotype analysis,individuals with the H2H4(CCTAGT)diplotype had signifi-cantly(P＜0.05)higher number of piglets born alive,primary litter weight,number of weaned piglets,and weaning litter weight compared to individuals with the H1H2(TCTAGT)diplotype and H2H2(CCAATT)diplotype.The H2H2(CCAATT)individuals had significantly(P＜0.05)higher average birth weight than individuals of other dip-lotypes except for H4H4(CCTTGG),and its average weaning weight was significantly(P＜0.05)higher than that of other diplotypes.The three mutation sites in intron 4 of CRISP3 gene were significantly associated with 6 reproduc-tive traits of Kole pigs,which could be used as candidate molecular markers for selection of reproductive traits in Kole pigs.
Kela pigCRISP3 genesingle nucleotide polymorphismreproductive trait
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