Effects of different contents of Camphora longepaniculata leaves in diets on intestinal pH,ce-cal fermentation and cecal microbiota of meat rabbits
In order to investigate the effects of different amounts of Camphora longepaniculata leaves in the diet on the intestinal pH,cecal fermentation and microbiota of meat rabbits,a total of 120 30-day-old New Zealand rabbits were fed with diets containing 0(group A),3％(group B),6％(group C),or 12％(group D)C.longepanicu-lata leaves.After 60 days,the pH in the intestinal tracts of meat rabbits was determined,and the volatile fatty acid contents and microbial diversity in the cecum were assessed by gas chromatography and high-throughput techniques,respectively.The results showed that the addition of C.longepaniculata leaves had no significant effect on intestinal pH and cecal volatile fatty acid content.Different amounts of C.longepaniculata leaves in the diet had significant(P＜0.05)effects on the number of bacterial and fungal operation taxonomic units(OTUs)in the cecum.The number of bacterial OTUs in group B was significantly lower than that in group C and group D,and the number of fungal OTUs in the group C was significantly higher than that in the other groups.Compared with the group A,the relative abundance of Bacteroidota in group B and C was significantly elevated,while the relative abundance of Verrucomicro-biota in group C and D,and Synergistota in group D was significantly decreased at the phylum level.At the genus level,the relative abundance of Anaerostipes,Bacteroides,Muribaculaceae unclassified,Marvinbryantia,Phascolarc-tobacterium,Blautia,Akkermansia and Synergistes in group B,and Ruminococcus in group D was significantly higher than that in group A.In conclusion,addition of C.longepaniculata leaves into dites had no adverse effects on the in-testinal pH,cecal fermentation and microbiota,and the addition of optimal amount of C.longepaniculata leaves(3％)could enhance the abundance of microflora in the cecum and promote the growth of probiotics.
Camphora longepaniculata leafmeat rabbitcecal fermentationmicrobial community structure
NETL
NSTL
万方数据
