金银花光形态建成因子LjCOP1基因克隆及表达模式分析
Cloning of light regulator LjCOP1 in Lonicera japonica and its expression characteristics
宛燕 1周晓春 2房海灵 1林沂 3亓希武 4于盱 4陈泽群 4梁呈元4
作者信息
- 1. 南京中医药大学,江苏南京 210023;江苏省中国科学院植物研究所,江苏南京 210014
- 2. 盐城市大丰区林场,江苏盐城 224100
- 3. 南京中医药大学,江苏南京 210023
- 4. 江苏省中国科学院植物研究所,江苏南京 210014
- 折叠
摘要
光是影响植物生长发育及次生代谢合成的最重要的环境因子之一,COP1 E3连接酶是光信号转导的分子开关.该研究基于金银花转录组数据库,从金银花(Lonicera japonica Thunb.)中克隆得到LjjCOP1基因,并对其进行生物信息学及表达模式分析.结果表明,该基因包含13个外显子,编码区(CDS)长度为2 034 bp,编码677个氨基酸残基.多序列比对显示,LjCOP1与其他植物COP1序列具有较高保守性,均包含环形锌指结合域、卷曲螺旋形和WD40重复序列等特殊结构域.基于SWISS-MODEL对金银花LjCOP1基因进行的蛋白结构预测显示其与拟南芥AtCOP1在蛋白质三级结构层面高度保守.启动子顺式作用元件分析表明,LjCOP1启动子序列中含有大量激素响应元件、光响应元件和胁迫响应元件.系统发生分析表明,LjCOP1与莴笋LsCOP1基因亲缘关系较近.表达模式分析显示,LjCOP1在金银花不同部位及花发育时期均有表达,且在叶中高量表达.与黑暗条件相比,白光下LjCOP1基因表达丰度最高,且对黑暗转蓝光响应更强烈.
Abstract
Light is one of the most important environmental factors to regulate growth,development and metabolism of plants,and COP 1(Constitutively photomorphogenic 1)is a molecular switch for light signal transduction.In this study,LjCOP1 gene was cloned from honeysuckle(Lonicera japonica Thunb.)based on the honeysuckle transcrip-tome database,and analyzed for its bioinformatics and expression pattern.The results showed that LjCOP1 contains 13 exons and 12 introns,with a coding sequence(CDS)length of 2 034 bp encoding 677 amino acid residues.Mul-tiple sequence comparison showed that LjCOP1 is highly conserved with other plant COP1 sequences,and all of them contain special structural domains such as ring zinc finger binding domain,coiled-coil domain and WD40 repeats.Protein structure prediction based on SWISS-MODEL showed that LjCOP1 was highly conserved with AtCOP1 of Ara-bidopsis thaliana at the level of protein tertiary structure.The analysis of promoter cis-acting progenitor showed that pLjCOP1 contained a large number of hormone-responsive,light-responsive,and stress-responsive elements.Phylo-genetic analysis showed that LjCOP1 and LsCOP1 in lettuce genes were closely related.Furthermore,expression pat-tern analysis showed that LjCOP1 was expressed in different parts of honeysuckle and during the period of flower de-velopment,and was highly expressed in leaves.Compared with the dark condition,the highest gene abundance of Lj-COP1 was found in white light,and responded more strongly to dark-to-blue light.
关键词
金银花/LjCOP1基因/表达模式Key words
Lonicera japonica/LjCOP1 gene/expression characteristic引用本文复制引用
基金项目
国家自然科学基金(32000262)
江苏省林业科技创新与推广项目(LYKJ[2021]20)
常州市科技支撑计划项目(CE20202006)
出版年
2024
NETL
NSTL
万方数据