Cloning of light regulator LjCOP1 in Lonicera japonica and its expression characteristics
Light is one of the most important environmental factors to regulate growth,development and metabolism of plants,and COP 1(Constitutively photomorphogenic 1)is a molecular switch for light signal transduction.In this study,LjCOP1 gene was cloned from honeysuckle(Lonicera japonica Thunb.)based on the honeysuckle transcrip-tome database,and analyzed for its bioinformatics and expression pattern.The results showed that LjCOP1 contains 13 exons and 12 introns,with a coding sequence(CDS)length of 2 034 bp encoding 677 amino acid residues.Mul-tiple sequence comparison showed that LjCOP1 is highly conserved with other plant COP1 sequences,and all of them contain special structural domains such as ring zinc finger binding domain,coiled-coil domain and WD40 repeats.Protein structure prediction based on SWISS-MODEL showed that LjCOP1 was highly conserved with AtCOP1 of Ara-bidopsis thaliana at the level of protein tertiary structure.The analysis of promoter cis-acting progenitor showed that pLjCOP1 contained a large number of hormone-responsive,light-responsive,and stress-responsive elements.Phylo-genetic analysis showed that LjCOP1 and LsCOP1 in lettuce genes were closely related.Furthermore,expression pat-tern analysis showed that LjCOP1 was expressed in different parts of honeysuckle and during the period of flower de-velopment,and was highly expressed in leaves.Compared with the dark condition,the highest gene abundance of Lj-COP1 was found in white light,and responded more strongly to dark-to-blue light.
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