Light is one of the most important environmental factors to regulate growth,development and metabolism of plants,and COP 1(Constitutively photomorphogenic 1)is a molecular switch for light signal transduction.In this study,LjCOP1 gene was cloned from honeysuckle(Lonicera japonica Thunb.)based on the honeysuckle transcrip-tome database,and analyzed for its bioinformatics and expression pattern.The results showed that LjCOP1 contains 13 exons and 12 introns,with a coding sequence(CDS)length of 2 034 bp encoding 677 amino acid residues.Mul-tiple sequence comparison showed that LjCOP1 is highly conserved with other plant COP1 sequences,and all of them contain special structural domains such as ring zinc finger binding domain,coiled-coil domain and WD40 repeats.Protein structure prediction based on SWISS-MODEL showed that LjCOP1 was highly conserved with AtCOP1 of Ara-bidopsis thaliana at the level of protein tertiary structure.The analysis of promoter cis-acting progenitor showed that pLjCOP1 contained a large number of hormone-responsive,light-responsive,and stress-responsive elements.Phylo-genetic analysis showed that LjCOP1 and LsCOP1 in lettuce genes were closely related.Furthermore,expression pat-tern analysis showed that LjCOP1 was expressed in different parts of honeysuckle and during the period of flower de-velopment,and was highly expressed in leaves.Compared with the dark condition,the highest gene abundance of Lj-COP1 was found in white light,and responded more strongly to dark-to-blue light.
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