Effects of NaCl on rooting,in-situ distribution of IAA and related enzyme activities of cherry rootstock seedlings
In order to clarify the effect of NaCl on the rooting of cherry rootstock,this experiment took the Gisela 6 tissue culture seedling as the material,and firstly studied the index of adventitious root occurrence in the tissue cul-ture seedling treated with different concentrations of NaCl.Then,the anatomical structure,enzyme activity and in-situ distribution of IAA under 10 mmol·L-1 and 80 mmol·L-1 NaCl were further studied according to rooting indexes.The results showed that the rooting rate and rooting index of cherry tissue seedlings were the highest under the treatment of 10 mmol·L-1 NaCl,and were significantly higher than other treatments,which were 58％and 13.43,respectively.Under this treatment the rooting speed was the fastest,the cambium thickened at 2 d,the root primodia formed at 6 d,and the adventitious roots broke through the epidermis at 10 d.SOD activity of each treatment reached the peak at 6 d.The activity changes of POD,PPO and IAAO were different among all treatments,while the activity of CK showed an increasing trend.Compared with CK,the activity of IAAO in 10 mmol·L-1 treatment peaked at 2 d.Using immune colloidal gold technique to analysis the in-situ distribution of IAA,we found under 10 mmol·L-1 NaCl treatment the IAA mainly distributed in cambium at 2 d,in root primordium at 6 d,and in the adventitious root vascular tissue at 10 d.In conclusion,10 mmol·L-1 NaCl treatment can promote adventitious roots of cherry tissue culture seedlings,and provide a reference for further research on the occurrence and regulation mechanism of adventitious roots.
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