Simultaneous determination of 15 quinolones in livestock and poultry excrement by UPLC-MS/MS
An analytical method for the simultaneous determination of 15 quinolones residues in livestock and poultry excrement by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was estab-lished.The sample was crushed and sieved after freeze-drying,extracted by mixing N,N-dimethylformamide(DMF)and 0.1 mol·L-1 hydrochloric acid solution,purified by HLB solid phase extraction column,detected by UPLC-MS/MS,and quantified by matrix matching calibration external standard method.The results showed that the linearity of 15 quinolones was good in the concentration range of 2-50 ng·mL-1,the coefficient of determination(R2)>0.99,and the detection limit was 5 μg·kg-1,limit of quantitation was 10 μg·kg-1,the average recovery rate was 76.2%-109.0%,relative standard derivation(RSD)was 1.3%-12.4%(n=6)at the concentration of 10,20 and 500 μg·kg-1.This method has achieved lower detection limit,quantitative limit,higher recovery rate and stability.It can be applied to the monitoring of quinolones residues in livestock and poultry excrement,provide accurate guidance for the scientific application of livestock and poultry excrement in the planting industry,provide technical methods for indirect monitoring of the content of quinolones in the breeding process,and guide the rational use of antibiotics by breeding enterprises.
UPLC-MS/MSquinoloneslivestock and poultry excrement
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