Effect of low-temperature stress on proline metabolism and ascorbic acid-glutathione cycle during germination of three Pueraria lobata germplasm lines
To investigate the effects of different temperature on proline metabolism and ascorbic acid-glutathione(AsA-GSH)circulatory system during germination of Pueraria lobata seeds,three lines of P.lobata collected from Australian(AUS),and Jiangsu Province(JS),Hunan Province(HN)in China,were subjected to germination tests by using the paper interval method to study the changes in proline content,key enzymes activities of proline me-tabolism,and contents of non-enzymatic antioxidants and activities of enzymatic antioxidants in the metabolism of AsA-GSH cycle under different treatments(CK,25℃;LT,15℃;LTI1,10℃;LTI2,seed soaking by 0.1%H2O2+10℃).The results showed that compared to the CK,low temperature stress increased contents of malondial-dehyde(MDA),hydrogen peroxide(H2O2)and decreased proline dehydrogenase(ProDH)activity.Application of H2O2 increased the contents of proline,ascorbic acid(AsA),dehydro ascorbic acid(DHA),glutathione(GSH),glutathione disulfide(GSSG),and activities of Δ1-pyrroline-5-carboxylic acid synthase(P5CS),ornithine amin-otransferase(OAT),ascorbate peroxidase(APX)and glutathione reductase(GR),hence improved the low-tem-perature resistance of P.lobata.Based on the membership function analysis,the three P.lobata germplasm lines were ranked as HN>AUS>JS in order of cold-tolerance.
Pueraria lobatalow temperature stressproline metabolism
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