Identification and functional analysis of CLE gene family in Dendrobium officinale Kimura et Migo
CLE peptide are a class of small secreted proteins that widely participate in plant development and cell-to-cell communication,playing a key regulatory role in maintaining the proliferation and differentiation of stem cells.In order to study the function of CLE family member in Dendrobium officinale Kimura et Migo,RNA was extracted from the leaves,roots,stems,buds and fruits of D.officinale and reverse transcribed into cDNA,and the expression of CLE family genes in each tissue was detected by semi-quantitative RT-PCR.The results showed that 17 CLE mem-bers were identified in D.officinale,all of which conserved 12-amino acid CLE motifs.Semi-quantitative RT-PCR performed on roots,stems,leaves,flowers and fruits showed that these CLE members had different tissue expression profiles,particularly CLE19635 with specifical expression in flower buds and fruits,and CLE22175 with specifical expression in fruits.In vitro synthesis of CLE peptides for treatment of Arabidopsis thaliana revealed that CLE pep-tides impacted the growth of A.thaliana roots,CLE05351 promoted root elongation,CLE18468 had no obvious effect,but the other CLE peptides all inhibited root elongation.mPS-PI staining did not find that the cell pattern of root-tip meristem was affected by CLE peptides.In addition,T2 homozygotes of A.thaliana overexpressing CLE02038 showed a short root phenotype,which was consistent with the result of CLE peptides treatment.The re-sults laid a foundation for the further exploring the role of CLE family in the epiphytic root of D.officinale and its sig-nal regulation network,and provided potential target genes for breeding of ideal root systems in D.officinale.
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