首页|氟吡菌酰胺在铁皮石斛中的残留与膳食风险

氟吡菌酰胺在铁皮石斛中的残留与膳食风险

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建立一种可同时检测氟吡菌酰胺及其代谢物 2-(三氯甲基)苯甲酰胺(BZM)在铁皮石斛(鲜样和干样)中残留量的分析方法,采用盆栽实验探究氟吡菌酰胺和BZM在铁皮石斛上的残留行为,评估氟吡菌酰胺的长期膳食风险.建立的检测方法为样品经乙腈提取后,用十八烷基硅烷键合硅胶、N-丙基乙二胺和石墨化碳净化,之后用超高效液相色谱-串联质谱法进行检测.在0.001～0.1 mg·L-1范围内,所建立的氟吡菌酰胺和BZM检测方法线性关系良好,决定系数(R2)≥0.998 1.在0.01、0.1、15 mg·kg-1的添加水平下,氟吡菌酰胺和BZM在铁皮石斛中的回收率分别为 85.71%～97.98%、94.14%～112.21%,相对标准偏差分别为0.39%～5.93%、1.22%～6.73%.41.7%的氟吡菌酰胺悬浮剂以推荐剂量(有效成分 375.3 g·hm-2)或 2倍推荐剂量施用1 次,氟吡菌酰胺在铁皮石斛鲜茎和干茎上的消解均符合一级动力学方程,消解半衰期为19.20～28.76 d,属于易降解农药.在实验条件下,施药后 75 d,氟吡菌酰胺和BZM在铁皮石斛中的残留量分别不超过1.98、0.09 mg·kg-1.经测算,氟吡菌酰胺的全膳食国家估算每日摄入量为0.380 6 mg,对应的风险商为60.41%,说明41.7%的氟吡菌酰胺悬浮剂在推荐剂量下施用于铁皮石斛上并不会对人体健康产生不可接受的风险.
Residues and dietary risk assessment of fluopyram in Dendrobium officinale
A method was established for the simultaneous determination of fluopyram and its metabolite 2-(triflu-oromethyl)benzamide(BZM)in both fresh and dried Dendrobium officinale.Pot experiment was conducted to inves-tigate the residue behaviors of fluopyram and BZM in D.officinale,and the long-term dietary risk of fluopyram was e-valuated.The established determination method was as follows:the samples were extracted with acetonitrile,purified with octadecyl bonded silica gel(C18),primary secondary amine(PSA)and PestiCarb(PC),and detected by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The result showed that fluopyram and BZM had linearities that ranged from0.001 to0.1 mg·L-1,with correlation coefficient≥0.998 1.The recover-ies of fluopyram and BZM in D.officinale at three spiked levels(0.01,0.1,15 mg·kg-1)were 85.71%-97.98%,94.14%-112.21%,respectively,with relative standard deviations of 0.39%-5.93%,1.22%-6.73%,respectively.When fluopyram was applied at recommended does(375.3 g·hm-2,based on the available in-gredient)or double recommended does to D.officinale,the dissipation dynamics of fluopyram fitted the first-order ki-netics equation,and the half-life of fluopyram in D.officinale was 19.20-28.76 d,indicating that fluopyram fell un-der the category of easily degradable pesticides.The residues of fluopyram and BZM in D.officinale did not exceed 1.98,0.09 mg·kg-1 after 75 days of application under the experiment contions.Calculation results showed that the na-tional estimated daily intake of fluopyram was 0.380 6 mg for the general population under the given conditions,and the corresponding dietary risk quotient was 60.41%,which indicated that applying 41.7%fluopyram suspension con-centrate(SC)at the recommended dosage on D.officinale would not pose an unacceptable risk to human health.

fluopyramDendrobium officinaleultra-performance liquid chromatography-tandem mass spectrometrypesticide residuedietary risk assessment

王晓梅、骆玉琴、赵学平、陆兰菲、方楠、王祥云、蒋金花、何红梅、张昌朋、王强

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中国计量大学 生命科学学院,浙江 杭州 310018

浙江省农业科学院 农产品质量安全与营养研究所,浙江 杭州 310021

氟吡菌酰胺 铁皮石斛 超高效液相色谱-串联质谱 农药残留 膳食风险评估

浙江省基础公益研究计划浙江省农业标准化生产示范创建("一县一品一策")项目

LGN21C140006ZJNY2022001-014

2024

10.3969/j.issn.1004-1524.20230479

浙江农业学报
浙江省农业科学院 浙江省农学会

浙江农业学报

CSTPCD北大核心
影响因子：0.765
ISSN：1004-1524
年,卷(期)：2024.36(7)
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