Cloning and expression analysis of Fagopyrum tataricum FtDELLA gene
DELLA protein is a negative regulatory protein in the gibberellin(GA)transduction pathway,which plays an important role in plant growth and development and stress response.In this experiment,a DELLA gene,named FtDELLA,was cloned from buckwheat using RT-PCR.The gene is 1 452 bp in CDS length and encodes 483 amino acids.Phylogenetic analysis showed that the FtDELLA protein contained the conserved DELLA conserved structural domains of the DELLA family.The qRT-PCR results showed that FtDELLA was highly expressed in stems and pre-grouting seeds,and its expression showed a decreasing trend during seed development.Under drought stress,the ex-pression of FtDELLA showed a decreasing trend,indicating that it may be a negative regulatory gene for drought stress response in buckwheat.The results suggest that FtDELLA may play an important regulatory role in the growth and development of buckwheat and drought stress response.The results provide a theoretical basis for further research on the function and mechanism of action of FtDELLA in buckwheat.
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