Identification of RcGeBP transcription factor and cloning and expression analysis of RcGeBP2 gene in castor
The GeBP gene family encodes a typical transcription factor that affects plant development and enhances plant survival in adverse environments by regulating the growth and differentiation of epidermal hairs.In order to ex-plore the distribution of GeBP gene in the castor genome and the function of the characteristic gene RcGeBP2,all the GeBP genes of castor were identified in this paper.The RcGeBP2 gene was cloned by RT-PCR and its molecular characteristics were analyzed,including bioinformatics,subcellular localization and expression pattern.The results showed that four RcGeBP genes were unevenly distributed on three chromosomes in the genome of castor.The pro-teins encoded by RcGeBP genes were hydrophilic proteins,and the advanced structure was mainly random coil.The phylogenetic tree construction of GeBP showed that the GeBP protein of castor had the closest relationship with the GeBP protein of tomato.The GeBP gene of castor had three and two genome duplications with the GeBP gene of toma-to and Arabidopsis thaliana,respectively.The results of Ka/Ks showed that the evolution of GeBP gene among spe-cies was passivated.The results of cis-acting element prediction showed that the RcGeBP promoter region contained multiple stress response and hormone-inducible elements.The FPKM expression value of RcGeBP genes showed that these genes had tissue expression characteristics and their expression was activated by drought and salt stress.The subcellular localization of RcGeBP2 protein showed that it was localized in the nucleus.In summary,this study has certain reference value for exploring the cross-regulation of castor RcGeBP gene family involved in salt and drought stress.
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